Product Details

Uniprot No.

Target Names

Alternative Names

cGMP-dependent 3',5'-cyclic phosphodiesterase (EC 3.1.4.17) (Cyclic GMP-stimulated phosphodiesterase) (CGS-PDE) (cGSPDE), PDE2A

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human PDE2A

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

11C6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IF

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200

Protocols

ELISA Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The PDE2A recombinant monoclonal antibody is carefully produced through a systematic process to ensure its quality and specificity. Initially, B cells are isolated from an immunized animal using 啊synthesized peptide derived from human PDE2A as the immunogen. Total RNA is then extracted from the harvested B cells, followed by reverse transcription to generate cDNA. The PDE2A antibody genes are amplified using PCR with primers specific to the antibody constant regions and inserted into an expression vector. This vector is subsequently transfected into host cells, allowing for the production of the PDE2A recombinant monoclonal antibody. The antibody is harvested from the cell culture supernatant and purified using affinity chromatography, resulting in a highly purified preparation. Extensive characterization assays, including ELISA and IF analysis, are performed to verify the antibody's specificity and functionality, ensuring its specific binding to human PDE2A protein.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Plays an important role in growth and invasion of malignant melanoma cells (e.g. pseudomyxoma peritonei (PMP) cell line).; Regulates mitochondrial cAMP levels and respiration. Involved in the regulation of mitochondria morphology/dynamics and apoptotic cell death via local modulation of cAMP/PKA signaling in the mitochondrion, including the monitoring of local cAMP levels at the outer mitochondrial membrane and of PKA-dependent phosphorylation of DNM1L.

Gene References into Functions

the well-known interaction between AIP and 2 different isoforms of phosphodiesterases (PDEs), PDE2A3 and PDE4A5, is of particular interest. While the interaction with over-expressed AIP does not seem to affect PDE2A3 function, the reported effect on PDE4A5 is, in contrast, reduced enzymatic activity. PMID: 28427099
Lu AF33241 represents a novel PDE2A/PDE10A inhibitor tool which can penetrate the blood brain barrier. PMID: 25941078
Epigenetic regulation of phosphodiesterases 2A and 3A underlies compromised beta-adrenergic Signaling in an induced pluripotent stem cell model of dilated cardiomyopathy. PMID: 26095046
curcumin exerts its in vitro anti-angiogenic and in vivo anti-tumour properties through combined PDE2 and PDE4 inhibition PMID: 25230992
PDE2A is involved in growth and invasion of human malignant melanoma PMP cells. PMID: 24705027
This study reports the X-ray crystal structure of PDE2A in complex with a highly selective, nanomolar inhibitor (BAY60-7550) at 1.9 A resolution, and the structure of apo PDE2 at 2.0 A resolution. PMID: 23899287
PDE2 overexpression in healthy cardiomyocytes reduces the rise in cyclic AMP levels and L-type calcium current amplitude, and abolishes the inotropic effect following acute beta-adrenergic receptor stimulation. PMID: 23810893
Our results demonstrated that the PDE2A2 variant carrying point mutations is expressed in PMP cells and may affect cell cycle progression by modulating cyclin A expression. PMID: 23381931
found in smooth muscle wall of blood vessels transversing the clitoral supepithelial and stromal space PMID: 21697861
Active site similarity between human and Plasmodium falciparum phosphodiesterases: considerations for antimalarial drug design PMID: 21766240
the phosphodiesterase 2A isoform localized to mitochondria regulates respiration PMID: 21724846
Inhibition of PDE2 resulted in higher levels of intracellular cAMP than inhibition of PDE3A suggesting this PDE may be the more important regulator of cAMP in human platelets. PMID: 12038792
TNF-alpha-mediated up-regulation of PDE2 may destabilize endothelial barrier function in sepsis PMID: 15650061
Phosphodiesterase 2 mediates redox-sensitive endothelial cell proliferation and angiogenesis by thrombin via Rac1 and NADPH oxidase 2. PMID: 19390057
report the X-ray crystal structures of a PDE protein that includes both catalytic and regulatory domains, namely, PDE2A containing the N-terminal GAF domains and the catalytic domain. PMID: 19828435

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Subcellular Location

[Isoform PDE2A3]: Cell membrane; Lipid-anchor.; [Isoform PDE2A2]: Mitochondrion matrix. Mitochondrion inner membrane. Mitochondrion outer membrane.; [Isoform PDE2A1]: Cytoplasm.; [Isoform 5]: Mitochondrion.

Protein Families

Cyclic nucleotide phosphodiesterase family, PDE2 subfamily

Tissue Specificity

Expressed in brain and to a lesser extent in heart, placenta, lung, skeletal muscle, kidney and pancreas.

Database Links

HGNC: 8777

OMIM: 602658

KEGG: hsa:5138

STRING: 9606.ENSP00000334910

UniGene: Hs.503163

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Code	CSB-RA150831A0HU
Size	US$210
	Order now
Image	Immunofluorescence staining of HepG2 cell with CSB-RA150831A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 569-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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PDE2A Recombinant Monoclonal Antibody

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Target Background