PIF1 Antibody

Datasheet

Code	CSB-PA863987LA01HU
Size	US$166
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Image	Immunohistochemistry of paraffin-embedded human gastric cancer using CSB-PA863987LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA863987LA01HU at dilution of 1:100
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PIF1 Polyclonal antibody

Uniprot No.

Q9H611

Target Names

PIF1

Alternative Names

ATP dependent DNA helicase PIF1 antibody; ATP-dependent DNA helicase PIF1 antibody; C15orf20 antibody; DNA helicase homolog PIF1 antibody; DNA helicase PIF1 antibody; DNA repair and recombination helicase PIF1 antibody; Petite integration frequency 1 antibody; PIF antibody; PIF1 5' to 3' DNA helicase antibody; PIF1 5' to 3' DNA helicase homolog antibody; PIF1 antibody; PIF1 DNA helicase antibody; PIF1/RRM3 DNA helicase like protein antibody; PIF1/RRM3 DNA helicase-like protein antibody; PIF1_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human ATP-dependent DNA helicase PIF1 protein (125-360AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The PIF1 Antibody (Product code: CSB-PA863987LA01HU) is Non-conjugated. For PIF1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA863987LB01HU	PIF1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA863987LC01HU	PIF1 Antibody, FITC conjugated
Biotin	CSB-PA863987LD01HU	PIF1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Possesses an intrinsic strand annealing activity.

Gene References into Functions

Structural and functional characterization of the unwinding mechanism of Bacteroides and human Pif1 helicases has been reported. PMID: 26904952
Authors characterised a functional role for human PIF1 in DNA replication that becomes important for cell growth under oncogenic stress. PMID: 25359767
Pif1 readily unfolds a parallel quadruplex DNA substrate in a multiturnover reaction and also generates some product under single cycle conditions PMID: 25589786
A minimum of 10 oligonucleotide bases are required for PIF1 binding and the hydrolysis of ATP. PMID: 23924759
the yeast ortholog of Pif1 appears to move along DNA in single nucleotide or base pair steps, powered by hydrolysis of 1 molecule of ATP. PMID: 23596008
Evidence that the PIF1 N-terminal (PINT) domain as crucial functions in PIF1 helicase. PMID: 18835853
when expressed in yeast, human PIF1 suppressed both G-quadruplex-associated DNA damage and telomere lengthening PMID: 23657261
PIF1 variant L319P was identified in three breast cancer families PMID: 22347400
Findings suggest roles for PIF1 in S-phase entry and progression that are essential to protect human tumor cells from apoptosis. PMID: 21616935
Human Pif1 could have a role in processing G4 structures that arise in the single-stranded nucleic acid intermediates formed during DNA replication and gene expression. PMID: 20524933
hPif1 may regulate telomere elongation by decreasing telomerase processivity, possibly via a mechanism that involves the unwinding of telomerase RNA from telomeric DNA by hPif1. PMID: 16522649
Human PIF, like S. cerevisiae Pif1p, plays a role in telomerase regulation. PMID: 17172855
hPif1 in the nucleus may be involved in chromosome maintenance in association with DNA replication PMID: 17827721
hPif1 specifically recognizes and unwinds DNA structures resembling putative stalled replication forks. PMID: 19700773

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Subcellular Location

Nucleus.; [Isoform 4]: Mitochondrion.

Protein Families

Helicase family, PIF1 subfamily

Tissue Specificity

Weak ubiquitous expression.

Database Links

HGNC: 26220

OMIM: 610953

KEGG: hsa:80119

STRING: 9606.ENSP00000268043

UniGene: Hs.112160

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Service

Phage Display Service

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Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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