PIP4K2C Antibody

Datasheet

Code	CSB-PA819455LA01HU
Size	US$166
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Image	IHC image of CSB-PA819455LA01HU diluted at 1:200 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA819455LA01HU diluted at 1:200 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of U251 cells with CSB-PA819455LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PIP4K2C Polyclonal antibody

Uniprot No.

Q8TBX8

Target Names

PIP4K2C

Alternative Names

Phosphatidylinositol 4 phosphate 5 kinase antibody; Phosphatidylinositol 4 phosphate 5 kinase type II gamma antibody; Phosphatidylinositol-5-phosphate 4-kinase type II gamma antibody; Phosphatidylinositol-5-phosphate 4-kinase type-2 gamma antibody; PI(5)P 4-kinase type II gamma antibody; PI42C_HUMAN antibody; Pip4k2c antibody; PIP4KII-gamma antibody; PIP5K2C antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma protein (127-408AA )

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The PIP4K2C Antibody (Product code: CSB-PA819455LA01HU) is Non-conjugated. For PIP4K2C Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA819455LB01HU	PIP4K2C Antibody, HRP conjugated	ELISA
FITC	CSB-PA819455LC01HU	PIP4K2C Antibody, FITC conjugated
Biotin	CSB-PA819455LD01HU	PIP4K2C Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Phosphatidylinositol 5-phosphate 4-kinase with low enzymatic activity. May be a GTP sensor, has higher GTP-dependent kinase activity than ATP-dependent kinase activity. PIP4Ks negatively regulate insulin signaling through a catalytic-independent mechanism. They interact with PIP5Ks and suppress PIP5K-mediated PtdIns(4,5)P2 synthesis and insulin-dependent conversion to PtdIns(3,4,5)P3.

Subcellular Location

Endoplasmic reticulum. Cytoplasm.

Database Links

HGNC: 23786

KEGG: hsa:79837

STRING: 9606.ENSP00000347032

UniGene: Hs.745011

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Regulation of actin cytoskeleton Phosphatidylinositol signaling system

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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