PLD4 Antibody

Code CSB-PA839298LA01HU
Size US$166
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Image
  • Western blot
    All lanes: PLD4 antibody at 4µg/ml
    Lane 1: THP-1 whole cell lysate
    Lane 2: Human placenta tissue
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 56 kDa
    Observed band size: 56 kDa

  • Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA839298LA01HU at dilution of 1:100

  • Immunofluorescent analysis of A549 cells using CSB-PA839298LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) PLD4 Polyclonal antibody
Uniprot No.
Target Names
PLD4
Alternative Names
C14orf175 antibody; Choline phosphatase 4 antibody; EC 3.1.4.4 antibody; Phosphatidylcholine hydrolyzing phospholipase D4 antibody; Phosphatidylcholine-hydrolyzing phospholipase D4 antibody; Phospholipase D family member 4 antibody; Phospholipase D4 antibody; PLD 4 antibody; PLD4 antibody; PLD4_HUMAN antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Phospholipase D4 protein (52-506AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The PLD4 Antibody (Product code: CSB-PA839298LA01HU) is Non-conjugated. For PLD4 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA839298LB01HU PLD4 Antibody, HRP conjugated ELISA
FITC CSB-PA839298LC01HU PLD4 Antibody, FITC conjugated
Biotin CSB-PA839298LD01HU PLD4 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The preparation of the PLD4 polyclonal antibody involved immunizing rabbits with recombinant human phospholipase D4 protein (amino acids 52-506). The antibody was then purified using protein G affinity chromatography, resulting in a purity of over 95%. It shows reactivity with human PLD4a protein.

The PLD4 antibody has undergone testing in multiple applications, including ELISA, WB, IHC, and IF. It is suitable for detecting and quantifying PLD4a protein, investigating its protein-protein interactions, and visualizing its localization. This PLD4 antibody appears to be a valuable tool for studying the properties and functions of the PLD4a protein in various biological contexts.

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Target Background

Function
5'->3' DNA exonuclease which digests single-stranded DNA (ssDNA). Regulates inflammatory cytokine responses via the degradation of nucleic acids, by reducing the concentration of ssDNA able to stimulate TLR9, a nucleotide-sensing receptor. Involved in phagocytosis of activated microglia.
Gene References into Functions
  1. PLD4 expression in colon cancer tissues PMID: 27840999
  2. We determined that PLD4 is a novel susceptibility gene for sysetmic sclerosis in Japanese, thus confirming the involvement of PLD4 in autoimmunity. PMID: 23124809
Subcellular Location
Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatus, trans-Golgi network membrane; Single-pass type II membrane protein. Nucleus. Early endosome. Cytoplasmic vesicle, phagosome.
Protein Families
Phospholipase D family
Database Links

HGNC: 23792

KEGG: hsa:122618

STRING: 9606.ENSP00000376372

UniGene: Hs.407101

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