PLIN5 Antibody

Datasheet

Code	CSB-PA804867LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: MCF7 whole cell lysate, 293 whole cell lysate, Mouse Brain tissue lysate All lanes: PLIN5 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 51, 55 kDa Observed band size: 55 , 20, 25kDa IHC image of CSB-PA804867LA01HU diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody IHC image of CSB-PA804867LA01HU diluted at 1:300 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody IHC image of CSB-PA804867LA01HU diluted at 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PLIN5 Polyclonal antibody

Uniprot No.

Q00G26

Target Names

PLIN5

Alternative Names

Lipid storage droplet protein 5 antibody; LSDA5 antibody; MLDP antibody; OXPAT antibody; Perilipin 5 antibody; Perilipin-5 antibody; PLIN5 antibody; PLIN5_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from human PLIN5

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The PLIN5 Antibody (Product code: CSB-PA804867LA01HU) is Non-conjugated. For PLIN5 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA804867LB01HU	PLIN5 Antibody, HRP conjugated	ELISA
FITC	CSB-PA804867LC01HU	PLIN5 Antibody, FITC conjugated
Biotin	CSB-PA804867LD01HU	PLIN5 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:3000
IHC	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The PLIN5 polyclonal antibody is inducibly produced in the rabbit immunized with the recombinant human PLIN5 (305-453aa). It exists as the IgG isotype and liquid form. And it is purified by protein G affinity chromatography and reaches up to 95% in purity. This PLIN5 antibody can react with human- and mouse-origin PLIN5 proteins. It can recognize and detect the PLIN5 protein in three applications, including ELISA, WB, and IF.

Its target protein PLIN5, also known as OXPAT, mainly regulates lipid metabolism, particularly fatty acid oxidation, in different tissues. Its role in energy metabolism and glucose homeostasis suggests that PLIN5 may have important implications for metabolic diseases, such as obesity, diabetes, and cardiovascular disease.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Lipid droplet-associated protein that maintains the balance between lipogenesis and lipolysis and also regulates fatty acid oxidation in oxidative tissues. Recruits mitochondria to the surface of lipid droplets and is involved in lipid droplet homeostasis by regulating both the storage of fatty acids in the form of triglycerides and the release of fatty acids for mitochondrial fatty acid oxidation. In lipid droplet triacylglycerol hydrolysis, plays a role as a scaffolding protein for three major key lipolytic players: ABHD5, PNPLA2 and LIPE. Reduces the triacylglycerol hydrolase activity of PNPLA2 by recruiting and sequestering PNPLA2 to lipid droplets. Phosphorylation by PKA enables lipolysis probably by promoting release of ABHD5 from the perilipin scaffold and by facilitating interaction of ABHD5 with PNPLA2. Also increases lipolysis through interaction with LIPE and upon PKA-mediated phosphorylation of LIPE.

Gene References into Functions

The data highlight a key role of PLIN5 in lipid droplets function, first by finely adjusting lipid droplets fatty acids supply to mitochondrial oxidation, and second acting as a protective factor against lipotoxicity in skeletal muscle. PMID: 27922115
Notch1 expression is reduced and glucose-6-phosphatase and perilipin-5 (G6PC/PLIN5) are upregulated in liver biopsies from nonalcoholic steatohepatitis (NASH) and nonalcoholic fatty liver disease (NAFLD) patients. PMID: 27428080
High oxygen consumption in middle-aged men was reflected in higher perilipin 5 expression in skeletal muscle. PMID: 26447519
PLIN5 was significantly colocated with ATGL, mitochondria and CGI-58, indicating a close association between the key lipolytic effectors in resting skeletal muscle. PMID: 25054327
Perilipin 5 as a lipid droplet protein adapted to mitochondrial energy utilization PMID: 24535284
Sprint interval and traditional endurance training increase net intramuscular triglyceride breakdown and expression of perilipin 2 and 5 PMID: 23129790
PLIN5 likely plays an important role in intramyocellular lipid accumulation and oxidation, both of which increase with endurance training in human skeletal muscle. PMID: 22667335
The lipid droplet coat protein perilipin 5 also localizes to muscle mitochondria. PMID: 22127648
Perilipin 5 is the most recently established family member, highly expressed in oxidative tissues and could play an important role in regulating LD TAG hydrolysis in oxidative mammalian tissues. [Review] PMID: 21632259
interaction of ATGL with CGI-58 increased lipolysis, whereas interaction of ATGL with perilipin 5 decreased lipolysis. PMID: 21393244
Results indicate involvement of OXPAT and ADRP in muscular lipid accumulation and type 2 diabetes. PMID: 19602560

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Subcellular Location

Lipid droplet. Cytoplasm. Mitochondrion.

Protein Families

Perilipin family

Tissue Specificity

Expressed in skeletal muscle, liver, heart and kidney.

Database Links

HGNC: 33196

OMIM: 613248

KEGG: hsa:440503

STRING: 9606.ENSP00000371272

UniGene: Hs.131034

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

PPAR signaling pathway

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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