POLR2G Antibody

Datasheet

Code	CSB-PA018334LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: A549 whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate All lanes: POLR2G antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 20 kDa Observed band size: 20 kDa
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) POLR2G Polyclonal antibody

Uniprot No.

P62487

Target Names

POLR2G

Alternative Names

DNA directed RNA polymerase II 19 kDa polypeptide antibody; DNA directed RNA polymerase II subunit G antibody; DNA directed RNA polymerase II subunit RPB7 antibody; DNA-directed RNA polymerase II subunit G antibody; DNA-directed RNA polymerase II subunit RPB7 antibody; hRPB19 antibody; hsRPB7 antibody; MGC138367 antibody; MGC138369 antibody; POLR2G antibody; polymerase (RNA) II (DNA directed) polypeptide G antibody; RNA polymerase II 19 kDa subunit antibody; RNA polymerase II subunit B7 antibody; RPB19 antibody; RPB7 antibody; RPB7_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human DNA-directed RNA polymerase II subunit RPB7 protein (1-172AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The POLR2G Antibody (Product code: CSB-PA018334LA01HU) is Non-conjugated. For POLR2G Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA018334LB01HU	POLR2G Antibody, HRP conjugated	ELISA
FITC	CSB-PA018334LC01HU	POLR2G Antibody, FITC conjugated
Biotin	CSB-PA018334LD01HU	POLR2G Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA. Binds RNA.

Gene References into Functions

The function of Rpb7 interaction with rpb4 in human cells indicate that Rpb7 have gene-specific effects but are also more generally required for human cell survival. PMID: 23073835
Identification of the RNA binding region of Rpb7. PMID: 16282592
the emerging transcript cross-links to the Rpb7 subunit of RNA polymerase II in various complexes containing 26- to 32-nt transcripts PMID: 16327806
TFIIF and Rpb7 are involved in both early and late transcriptional stages PMID: 17848138

Subcellular Location

Nucleus.

Protein Families

Eukaryotic RPB7/RPC8 RNA polymerase subunit family

Database Links

HGNC: 9194

OMIM: 602013

KEGG: hsa:5436

STRING: 9606.ENSP00000301788

UniGene: Hs.14839

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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