PPM1K Antibody

Datasheet

Code	CSB-PA822697LA01HU
Size	$600
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Image	Western Blot Positive WB detected in: COLO205 whole cell lysate(30µg), 293 whole cell lysate(30µg), A549 whole cell lysate(30µg), JK whole cell lysate(30µg), K562 whole cell lysate(30µg), Hela whole cell lysate(30µg),Mouse skeletal muscle tissue lysate(30µg) All lanes: PPM1K antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution Predicted band size: 41,26,17 kDa Observed band size: 41 kDa Exposure time: 120s IHC image of CSB-PA822697LA01HU diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody IHC image of CSB-PA822697LA01HU diluted at 1:100 and staining in paraffin-embedded human colorecta cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PPM1K Polyclonal antibody

Uniprot No.

Q8N3J5

Target Names

PPM1K

Alternative Names

BDP antibody; mitochondrial antibody; MSUDMV antibody; PP2C domain containing protein phosphatase 1K antibody; PP2C domain-containing protein phosphatase 1K antibody; PP2C kappa antibody; PP2C like mitochondrial protein antibody; PP2C type mitochondrial phosphoprotein phosphatase antibody; PP2C-kappa antibody; PP2C-like mitochondrial protein antibody; PP2C-type mitochondrial phosphoprotein phosphatase antibody; PP2Ckappa antibody; PP2CM antibody; PPM1K antibody; PPM1K_HUMAN antibody; Protein phosphatase 1K antibody; Protein phosphatase 1K; mitochondrial antibody; Protein phosphatase 2C isoform kappa antibody; Protein phosphatase Mg2+/Mn2+ dependent 1K antibody; PTMP antibody; UG0882E07 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Protein phosphatase 1K, mitochondrial protein (30-372AA)

Immunogen Species

Homo sapiens (Human)

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.02% sodium azide
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:100-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Regulates the mitochondrial permeability transition pore and is essential for cellular survival and development.

Gene References into Functions

Branched chain amino acids related genetic variant rs1440581 was associated with an increased risk of incident T2 Diabetes in a Chinese population. This variant might modify effect of weight gain on development in Insulin resistance. PMID: 29855804
PPM1K rs1440581 may affect changes in glucose metabolism during weight loss, and this effect is dependent on dietary fat and carbohydrate intakes. PMID: 28768654
miR-204 and miR-211 directly repress PPM1K expression via 3'UTR. PMID: 26592513
Overweight people with the C allele of the branched-chain amino acid/aromatic amino acid ratio-associated variant rs1440581 may benefit less in weight loss and improved insulin sensitivity than those without it on a energy-restricted high-fat diet. PMID: 23446828
The 2.4 A PP2Cm structure reveals a central beta-sandwich with two bound metal ions in the active-site cleft. PMID: 22291014
A novel member of human protein phosphatase 2C gene family named PP2Ckappa was isolated from a human fetal brain cDNA library. PMID: 16685424
The cloned phosphatase has a mitochondrial leader sequence and its amino acid sequence places it in the PP2C family like two known mitochondrial phosphatases. PMID: 17336929

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Involvement in disease

Maple syrup urine disease, mild variant (MSUDMV)

Subcellular Location

Mitochondrion matrix.

Protein Families

PP2C family

Database Links

HGNC: 25415

OMIM: 611065

KEGG: hsa:152926

STRING: 9606.ENSP00000295908

UniGene: Hs.43744

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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