PSMC4 Antibody

Datasheet

Code	CSB-PA018894LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Mouse liver tissue All lanes: PSMC4 antibody at 3.4μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 48, 44 kDa Observed band size: 48 kDa IHC image of CSB-PA018894LA01HU diluted at 1:500 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA018894LA01HU diluted at 1:500 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PSMC4 Polyclonal antibody

Uniprot No.

P43686

Target Names

PSMC4

Alternative Names

26S protease regulatory subunit 6B antibody; 26S proteasome AAA ATPase subunit RPT3 antibody; 26S proteasome AAA-ATPase subunit RPT3 antibody; MB67 interacting protein antibody; MB67-interacting protein antibody; MIP224 antibody; Protease 26S subunit 6 antibody; Proteasome (prosome macropain) 26S subunit ATPase 4 antibody; Proteasome 19S S6 antibody; Proteasome 26S subunit ATPase 4 antibody; Proteasome 26S subunit; ATPase; 4 antibody; PRS6B_HUMAN antibody; PSMC4 antibody; RPT3 antibody; S6 antibody; Tat binding protein 7 antibody; TAT-binding protein 7 antibody; TBP 7 antibody; TBP-7 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human 26S proteasome regulatory subunit 6B protein (112-181AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The PSMC4 Antibody (Product code: CSB-PA018894LA01HU) is Non-conjugated. For PSMC4 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA018894LB01HU	PSMC4 Antibody, HRP conjugated	ELISA
FITC	CSB-PA018894LC01HU	PSMC4 Antibody, FITC conjugated
Biotin	CSB-PA018894LD01HU	PSMC4 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC4 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.

Gene References into Functions

This study demonstrated that particular distribution for HSC70 and PSMC4 in the cytoplasm and accumulation within Lewy body in the dopaminergic neurons of the substantia nigra in Parkinson patients. PMID: 29218503
Structural mechanism for nucleotide-driven remodeling of RPT3 and its homologs in the activated human 26S proteasome has been reported. PMID: 29636472
Site-specific Rpt3 phosphorylation by DYRK2 controls cell proliferation and tumorigenesis in breast neoplasms. PMID: 26655835
Saquinavir-NO inhibits activation of S6 protein in androgen-dependent prostate cancer cells. PMID: 22370480
The proposed TRAP1 network has an impact in vivo, as it is conserved in human colorectal cancers, is controlled by ER-localized TRAP1 interacting with TBP7 and provides a novel model of the ER-mitochondria crosstalk. PMID: 21979464
C terminus of Rpt3, an ATPase subunit of PA700 (19 S) regulatory complex, is essential for 26 S proteasome assembly but not for activation PMID: 20937828
interacts with gankyrin through C-terminal 78 amino acids PMID: 11779854
a novel specific interaction of synphilin-1 with the regulatory proteasomal protein S6 ATPase (tbp7) in aggresome-like intracytoplasmic inclusions PMID: 17327361
An insertion/deletion variant in intron 5 of the S6 ATPase gene was more frequent in German Parkinson's disease patients compared to controls PMID: 18446261

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Subcellular Location

Cytoplasm. Nucleus.

Protein Families

AAA ATPase family

Database Links

HGNC: 9551

OMIM: 602707

KEGG: hsa:5704

STRING: 9606.ENSP00000157812

UniGene: Hs.211594

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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