PSMD2 Antibody

Datasheet

Code	CSB-PA619755LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, U251 whole cell lysate All lanes: PSMD2 antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 101, 83, 86 kDa Observed band size: 101, 40 kDa IHC image of CSB-PA619755LA01HU diluted at 1:1000 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA619755LA01HU at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PSMD2 Polyclonal antibody

Uniprot No.

Q13200

Target Names

PSMD2

Alternative Names

26S proteasome non-ATPase regulatory subunit 2 antibody; 26S proteasome regulatory subunit RPN1 antibody; 26S proteasome regulatory subunit S2 antibody; 26S proteasome subunit p97 antibody; 55.11 protein antibody; MGC14274 antibody; P97 antibody; Proteasome (prosome macropain) 26S subunit non ATPase 2 antibody; Proteasome 26S subunit; non-ATPase; 2 antibody; Proteasome 26S; subunit 2 antibody; Protein 55.11 antibody; Psmd2 antibody; PSMD2_HUMAN antibody; Rpn1 antibody; S2 antibody; TNFR associated protein 2 antibody; TRAP2 antibody; Tumor necrosis factor receptor associated protein 2 antibody; Tumor necrosis factor type 1 receptor-associated protein 2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human 26S proteasome non-ATPase regulatory subunit 2 protein (401-700AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The PSMD2 Antibody (Product code: CSB-PA619755LA01HU) is Non-conjugated. For PSMD2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA619755LB01HU	PSMD2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA619755LC01HU	PSMD2 Antibody, FITC conjugated
Biotin	CSB-PA619755LD01HU	PSMD2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:1000-1:2000
IF	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.; Binds to the intracellular domain of tumor necrosis factor type 1 receptor. The binding domain of TRAP1 and TRAP2 resides outside the death domain of TNFR1.

Gene References into Functions

the high-resolution solution structure of the UBL domain of human UBLCP1 and its interaction with Rpn1 PMID: 23667555
Results suggest that PSMD2 may be a good molecular target candidate. PMID: 21465578
These data suggest that p97 recruits proteasomes to polytopic ER proteins, such as Insig-1, even before they are extracted from membranes. PMID: 19815544
U87 glioblastoma cells transduced to express the epidermal growth factor receptor vIII (EGFRvIII) mutant do not respond toirrradiation with 26s proteasome inhibition. PMID: 18337449
Data show that data provide a novel mechanism whereby CaMKII may regulate the proteasome in neurons to facilitate remodeling of synaptic connections through protein degradation. PMID: 19638347
the 26S proteasome may provide a general predictive biomarker for radiotherapy outcome. PMID: 19881955

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Protein Families

Proteasome subunit S2 family

Tissue Specificity

Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta.

Database Links

HGNC: 9559

OMIM: 606223

KEGG: hsa:5708

STRING: 9606.ENSP00000310129

UniGene: Hs.518464

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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