PSMD7 Antibody

Datasheet

Code	CSB-PA018912LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: 293 whole cell lysate All lanes: PSMD7 antibody at 3.2μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 38 kDa Observed band size: 38 kDa IHC image of CSB-PA018912LA01HU diluted at 1:800 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of MCF-7 cells with CSB-PA018912LA01HU at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PSMD7 Polyclonal antibody

Uniprot No.

P51665

Target Names

PSMD7

Alternative Names

26S proteasome non ATPase regulatory subunit 7 antibody; 26S proteasome non-ATPase regulatory subunit 7 antibody; 26S proteasome regulatory subunit RPN8 antibody; 26S proteasome regulatory subunit S12 antibody; integration site gene; mouse; homolog of antibody; Moloney leukemia virus 34 proviral antibody; Moloney leukemia virus 34 proviral integration antibody; MOV34 antibody; Mov34 homolog antibody; Mov34 protein homolog antibody; MOV34L antibody; P40 antibody; Proteasome (prosome; macropain) 26S subunit; non ATPase 7 antibody; Proteasome (prosome; macropain) 26S subunit; non ATPase; 7 (Mov34 homolog) antibody; Proteasome 26S S12 antibody; Proteasome 26S subunit; no-ATPase; 7 antibody; Proteasome subunit p40 antibody; PSD7_HUMAN antibody; PSMD 7 antibody; PSMD7 antibody; Rpn8 antibody; S12 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human 26S proteasome non-ATPase regulatory subunit 7 protein (1-324AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The PSMD7 Antibody (Product code: CSB-PA018912LA01HU) is Non-conjugated. For PSMD7 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA018912LB01HU	PSMD7 Antibody, HRP conjugated	ELISA
FITC	CSB-PA018912LC01HU	PSMD7 Antibody, FITC conjugated
Biotin	CSB-PA018912LD01HU	PSMD7 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:500-1:1000
IF	1:50-1:500
IP	1:200-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.

Gene References into Functions

SNP rs17336700 of a non-MHC ubiquitin-proteasome subunit PSMD7 is significantly associated with ankylosing spondylitis in Chinese population and the mRNA level of the PSMD7 gene in liver tissue increased when the rs17336700 C allele was present. PMID: 20643764
hVIP is a member of the complex array of nucleocytoplasmic shuttling proteins that are regulated by HIV infection and glucocorticoids PMID: 12237292
data suggest a differential nuclear function of modified and unmodified S12 in cancer PMID: 15221960
MPN domain of Mov34 is highly resistant to proteolysis PMID: 16842755
although the MPN domain of Mov34 shows a typical metalloprotease fold, it is unable to coordinate a metal ion. PMID: 17559875

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Protein Families

Peptidase M67A family

Database Links

HGNC: 9565

OMIM: 157970

KEGG: hsa:5713

STRING: 9606.ENSP00000219313

UniGene: Hs.440604

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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