Phospho-EFNB1/EFNB2/EFNB3 (Tyr324) Antibody

Code CSB-PA142593
Size US$360
Image
  • Western blot analysis of extracts from K562 cells treated with serum using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody.The lane on the right is treated with the antigen-specific peptide.
  • Immunohistochemical analysis of paraffin-embedded human brain tissue using Ephrin B1/B2/B3 (Phospho-Tyr324) antibody (left)or the same antibody preincubated with blocking peptide (right).
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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) EFNB1/EFNB2/EFNB3 Polyclonal antibody
Uniprot No. P98172
Target Names EFNB1/EFNB2/EFNB3
Alternative Names CFND antibody; CFNS antibody; Craniofrontonasal syndrome (craniofrontonasal dysplasia) antibody; EFL 3 antibody; EFL-3 antibody; EFL3 antibody; EFNB1 antibody; EFNB1_HUMAN antibody; Elk L antibody; ELK ligand antibody; ELK-L antibody; Eph related receptor tyrosine kinase ligand 2 antibody; EPH-related receptor tyrosine kinase ligand 2 antibody; Ephrin-B1 antibody; EPLG2 antibody; LERK 2 antibody; LERK-2 antibody; LERK2 antibody; Ligand of eph related kinase 2 antibody; MGC8782 antibody
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Immunogen Peptide sequence around phosphorylation site of tyrosine 324 (G-D-Y(p)-G-H) derived from Human Ephrin B1/B2/B3 .
Immunogen Species Homo sapiens (Human)
Clonality Polyclonal
Purification Method Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Concentration It differs from different batches. Please contact us to confirm it.
Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Tested Applications ELISA,WB,IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:1000
IHC 1:50-1:100
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Binds to the receptor tyrosine kinases EPHB1 and EPHA1. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity).
Gene References into Functions
  1. Lymphomas with low UTX expression express high levels of Efnb1, and cause significantly poor survival. PMID: 30006524
  2. Chronic hypoxia-induced slug promotes invasive behavior of prostate cancer cells by activating the expression of ephrin-B1. PMID: 30058095
  3. This study showed that EphB2 cells have a transient increase in migration after heterotypic activation, which underlies a shift in the EphB2-ephrinB1 border but is not required for segregation or border sharpening. PMID: 28747399
  4. that expression of EFNB1 and EFNB2 is implicated in Th cell differentiation and migration to inflammatory sites in both EAE and MS PMID: 27039370
  5. we demonstrate that mosaicism for EPHRIN-B1 expression induced by random X inactivation in heterozygous females results in robust cell segregation in human neuroepithelial cells, thus supplying experimental evidence that Eph/ephrin-mediated cell segregation is relevant to pathogenesis in human CFNS patients. PMID: 28238796
  6. one novel (IVS2+3G>T) and one previously reported mutation (p.Gly151Ser) in EFNB1 Both patients were de novo cases without a family history of Craniofrontonasal syndrome. PMID: 27650623
  7. While ephrin-B1 deficiency leads to abnormal visual pathways in mice, it leaves the human visual system, apart from deficits in binocular vision, largely normal. PMID: 26580852
  8. that EphrinB1 (EFNB1) co-localizes with microtubules (MTs) during all phases of the cell cycle. PMID: 25436983
  9. we report a family with a G151S mutation in the EFNB1 gene. The mutation was identified in two severely affected sisters and paradoxically in their clinically unaffected father. PMID: 25486017
  10. T cells from rheumatoid arthritis (RA) patients expressed higher EFNB1 mRNA levels, which correlated with RA symptoms and laboratory findings. Expression of EFNB1 in T cells might be a parameter for monitoring RA disease activity and treatment responses. PMID: 25779027
  11. Results indicate that EphrinB1 is uniquely dysregulated in medulloblastoma and promotes oncogenic responses in medulloblastoma cells, implicating ephrinB1 as a potential target PMID: 25258252
  12. EphB2/ephrin-B1 were invoked in dental pulp stem cells with TNF-alpha treatment via the JNK-dependent pathway, but not NF-kB, p38 MAPK or MEK signalling. PMID: 25643922
  13. Patients with EFNB1 mutations have a clear phenotype. This study will facilitate genetic counseling of parents and patients, and contribute to the diagnostic and screening process of patients with suspected CFNS. PMID: 24281372
  14. CNK1 mediates ephrinB1 signaling that promotes cell migration through RhoA and JNK activity. PMID: 24825906
  15. EphrinB1 expression is related to the metastasis of breast cancer and its enhanced expression confers a poor prognosis, suggesting that EphrinB1 may be a relevant therapeutic target in breast cancers. PMID: 24240587
  16. High EFNB1 expression is associated with squamous cell carcinomas of the head and neck. PMID: 23811940
  17. we report the identification of mosaic EFNB1 mutations in every individual with craniofrontonasal syndrome, confirming the suggested diagnosis and supporting the hypothesis of cellular interference in humans. PMID: 23335590
  18. These results indicate a novel function of Nm23-H1 to control contact inhibition of locomotion, and its negative regulation by ephrin-B1. PMID: 22718351
  19. Data show that EphrinB1, a PTPN13 substrate, interacts with ErbB2, and Src kinase mediates EphrinB1 phosphorylation and subsequent MAP Kinase signaling. PMID: 22279592
  20. X-linked cases resembling Teebi hypertelorism may have a similar mechanism to CFNS, and that cellular mosaicism for different levels of ephrin-B1 (as well as simple presence/absence) leads to craniofacial abnormalities. PMID: 21542058
  21. Unreported mutation in EFNB1 predicts occurrence of congenital diaphragmatic hernia, phenotypic differences between males and female. PMID: 20734337
  22. The impact of craniofrontonasal syndrome-causing EFNB1 mutations on ephrin-B1 function is reported. PMID: 20565770
  23. study reports that the expression of ephrin-B1 is controlled by a feedback loop involving posttranscriptional regulatory mechanisms PMID: 20308325
  24. combination of SDF-1, PTN, IGF2, and EFNB1 mimics the DA phenotype-inducing property of SDIA and was sufficient to promote differentiation of hESC to functional midbrain DA neurons PMID: 19672298
  25. Human platelets express EphA4 and EphB1, and the ligand, ephrinB1. Forced clustering of EphA4 or ephrinB1 led to cytoskeletal reorganization, adhesion to fibrinogen, and alpha-granule secretion. PMID: 12084815
  26. Expression profile of this ligand of EPHB2 in gastric cancer PMID: 12136247
  27. The expression of ephrin-B1 was correlated with a poorer clinical prognosis. Ephrin-B1 protein was expressed by osteosarcoma cells and blood vessels. PMID: 12209731
  28. Results identified a novel interaction between EphB1 with the adaptor molecule Grb7 and suggested that this interaction may play a role in the regulation of cell migration by EphB1. PMID: 12223469
  29. expression of Eph-B1 in plasmacytoid dendritic cells PMID: 12384430
  30. Ephrin B1 induces human aortic endothelial cells migration in a pathway that involves Crk adaptor protein PMID: 12475948
  31. conclude that ephrinB1 and B class Eph receptors provide positional cues required for the normal morphogenesis of skeletal elements PMID: 12919674
  32. Eph/ephrin signaling enhances the ability of platelet agonists to cause aggregation by activating Rap1 and these effects require oligomerization of ephrinB1 but not phosphotyrosine-based interactions with the ephrinB1 cytoplasmic domain. PMID: 14576067
  33. Ephrin-B1 may be involved in in vivo tumor progression by promoting neovascularization in hepatocellular carcinoma PMID: 14642617
  34. Mutations of the ephrin-B1 gene cause craniofrontonasal syndrome PMID: 15124102
  35. ephrin-B1 internalization is an active receptor-mediated process that utilizes the clathrin-mediated endocytic pathway PMID: 15351694
  36. On ephrinB1 stimulation, the small GTPases Rho and Ras are activated and Rap1 is inactivated. PMID: 15725075
  37. EFNB1 mutations have a role in familial and sporadic craniofrontonasal syndrome (CFNS) PMID: 15959873
  38. EphB/ephrin-B molecules play a role in restricting dental pulp stem cell attachment and migration to maintain Dthesse cells within their stem cell niche under steady-state conditions. PMID: 17204606
  39. C-terminus of ephrin-B1 regulates activation of the extracellular release of MMP-8 without requirement of de novo protein synthesis. PMID: 17567680
  40. Our results suggest that tyrosine phosphorylation of ephrin-B1 promotes invasion of cancer cells in vivo PMID: 17591954
  41. Silencing of EphB expression is associated with colorectal tumorigenesis. PMID: 17906625
  42. EphrinB1 may play an important role in the inflammatory states of rheumatoid arthritis, by affecting the population/function of T cells. PMID: 17942634
  43. EFNB1 mutant transcripts with nonsense codons are degraded by the nonsense-mediated mRNA decay pathway and lead to craniofrontonasal syndrome. PMID: 18043713
  44. Ephrin-B1 is likely to play an important role in the regulation of malignant T lymphocytes through the control of lipid-raft-associated signaling, adhesion, and invasive activity PMID: 18314490
  45. Several new EFNB1 mutations have been identified in craniofrontonasal syndrome. PMID: 18627045

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Involvement in disease Craniofrontonasal syndrome (CFNS)
Subcellular Location Membrane, Single-pass type I membrane protein
Protein Families Ephrin family
Tissue Specificity Heart, placenta, lung, liver, skeletal muscle, kidney, pancreas.
Database Links

HGNC: 3226

OMIM: 300035

KEGG: hsa:1947

STRING: 9606.ENSP00000204961

UniGene: Hs.144700

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