Phospho-EFNB1/EFNB2/EFNB3 (Tyr324) Antibody

Datasheet
Code CSB-PA142593
Size US$360
Image
  • Western blot analysis of extracts from K562 cells treated with serum using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody.The lane on the right is treated with the antigen-specific peptide.
  • Immunohistochemical analysis of paraffin-embedded human brain tissue using Ephrin B1/B2/B3 (Phospho-Tyr324) antibody (left)or the same antibody preincubated with blocking peptide (right).

Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) EFNB1/EFNB2/EFNB3 Polyclonal antibody
Uniprot No. P98172
Target Names EFNB1/EFNB2/EFNB3
Alternative Names EPL2; CEL5-L; EFL-3; EFNB1; ELK-L
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Immunogen Peptide sequence around phosphorylation site of tyrosine 324 (G-D-Y(p)-G-H) derived from Human Ephrin B1/B2/B3 .
Immunogen Species Homo sapiens (Human)
Clonality Polyclonal
Purification Method Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Concentration It differs from different batches. Please contact us to confirm it.
Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Tested Applications ELISA,WB,IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:1000
IHC 1:50-1:100
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Target Data

Function Binds to the receptor tyrosine kinases EPHB1 and EPHA1. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity).
Involvement in disease Craniofrontonasal syndrome (CFNS)
Subcellular Location Membrane, Single-pass type I membrane protein
Protein Families Ephrin family
Tissue Specificity Heart, placenta, lung, liver, skeletal muscle, kidney, pancreas.
Database Links

HGNC: 3226

OMIM: 300035

KEGG: hsa:1947

STRING: 9606.ENSP00000204961

UniGene: Hs.144700

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