Phospho-HIST1H1B (T154) Antibody

Code CSB-PA010377PA154phHU
Size US$319
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate
    All lanes: HIST1H1B antibody at 1.5µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 23 kDa
    Observed band size: 32 kDa

  • Chromatin Immunoprecipitation Hela (4*106, treated with 100nM calyculin A for 60min) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H1B (CSB-PA010377PA154phHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

  • Immunofluorescence staining of HepG2 cells with CSB-PA010377PA154phHU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) HIST1H1B Polyclonal antibody
Uniprot No. P16401
Target Names HIST1H1B
Alternative Names H1 antibody; H1 histone family member 5 antibody; H1.5 antibody; H15 HUMAN antibody; H15_HUMAN antibody; H1B antibody; H1F5 antibody; H1s 3 antibody; Hist1h1b antibody; Histone 1 H1b antibody; Histone cluster 1 H1b antibody; Histone H1.5 antibody; Histone H1a antibody; Histone H1b antibody; Histone H1s 3 antibody; MGC126630 antibody; MGC126632 antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Peptide sequence around site of Phospho-Thr (154) derived from Human Histone H1.5
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Antigen Affinity Purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IF, ChIP
Recommended Dilution
Application Recommended Dilution
WB 1:200-1:2000
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Data

Function Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Gene References into Functions
  1. Data indicate that normal ovarian tissues strongly expressed histone H1.5, whereas ovarian granulosa cell tumors (GCTs) weakly expressed this protein; in contrast, PLZF protein expression was not significantly different between both study groups. PMID: 25023763
  2. Statistically significant differences in staining patterns were found for histone H1.5, distinguishing leiomyosarcomas from leiomyomas. PMID: 24784718
  3. Promyelocytic leukemia zinc finger and histone H1.5 differentially stain low- and high-grade pulmonary neuroendocrine tumors PMID: 23416030
  4. FoxP3 interacts with H1.5 to alter its binding to target genes to modulate their expression and to program Treg function. PMID: 21654845
  5. the mode of the chromatin fiber compaction changes depending both on salt environment and linker histone H1 PMID: 16185066
  6. Phosphorylation of human H1 variants occurs nonrandomly during both interphase and mitosis and distinct serine- or threonine-specific kinases are involved in different cell cycle phases. PMID: 16377619
  7. Phosphorylation at threonine 10 appears in prometaphase and disappears in telophase, and that this hyperphosphorylated form of H1.5 is mainly chromatin-bound in metaphase when chromatin condensation is maximal. PMID: 19136008

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Subcellular Location Nucleus, Chromosome
Protein Families Histone H1/H5 family
Tissue Specificity Ubiquitous. Expressed in the majority of the cell lines tested and in testis.
Database Links

HGNC: 4719

OMIM: 142711

KEGG: hsa:3009

STRING: 9606.ENSP00000330074

UniGene: Hs.131956

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