Phospho-HIST1H1B (T154) Antibody

Datasheet
Code CSB-PA010377PA154phHU
Size US$319
Uniprot No. P16401
Abbreviation Histone H1.5
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate
    All lanes: HIST1H1B antibody at 1.5ug/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 23 kDa
    Observed band size: 32 kDa

  • Chromatin Immunoprecipitation Hela(4*106, treated with 100nM calyculin A for 60min)were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H1B(CSB-PA010377PA154phHU)or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers against the β-Globin promoter.

  • Immunofluorescence staining of HepG2 cells with CSB-PA010377PA154phHU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunogen Peptide sequence around site of phospho-Thr (154) derived from human Histone H1.5.
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IF, ChIP; Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200
Relevance Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method Antigen Affinity Purified
Isotype IgG
Clonality Polyclonal
Alias Histone H1.5 (Histone H1a) (Histone H1b) (Histone H1s-3), HIST1H1B, H1F5
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Research Area Epigenetics and Nuclear Signaling
Target Names HIST1H1B
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Function Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Subcellular location Nucleus, Chromosome

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