Phospho-HIST1H1B (T154) Antibody

Datasheet

Code	CSB-PA010377PA154phHU
Size	US$175
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Image	IHC image of CSB-PA010377PA154phHU diluted at 1:50 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) HIST1H1B Polyclonal antibody

Uniprot No.

P16401

Target Names

HIST1H1B

Alternative Names

H1 antibody; H1 histone family member 5 antibody; H1.5 antibody; H15 HUMAN antibody; H15_HUMAN antibody; H1B antibody; H1F5 antibody; H1s 3 antibody; Hist1h1b antibody; Histone 1 H1b antibody; Histone cluster 1 H1b antibody; Histone H1.5 antibody; Histone H1a antibody; Histone H1b antibody; Histone H1s 3 antibody; MGC126630 antibody; MGC126632 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Peptide sequence around site of Phospho-Thr (154) derived from Human Histone H1.5

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

CUSABIO's product CSB-PA010377PA154phHU is a polyclonal antibody raised in rabbits against the synthesized peptide derived from human Histone H1.5 around the phosphorylation T154. This Phospho-HIST1H1B (T154) antibody occurs as an unconjugated IgG. It is only reactive with phosphorylated Histone H1.5 of human-origin. It does not cross-react with non-phosphorylated Histone H1.5 or other phosphorylated forms of Histone H1.5. Validations in ELISA, WB, IF, and ChIP applications of this antibody have been done. The target protein Histone H1.5 is involved in several biological processes, such as cell differentiation, nucleosome spacing and mRNA splicing, and tumorigenesis.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.

Gene References into Functions

Data indicate that normal ovarian tissues strongly expressed histone H1.5, whereas ovarian granulosa cell tumors (GCTs) weakly expressed this protein; in contrast, PLZF protein expression was not significantly different between both study groups. PMID: 25023763
Statistically significant differences in staining patterns were found for histone H1.5, distinguishing leiomyosarcomas from leiomyomas. PMID: 24784718
Promyelocytic leukemia zinc finger and histone H1.5 differentially stain low- and high-grade pulmonary neuroendocrine tumors PMID: 23416030
FoxP3 interacts with H1.5 to alter its binding to target genes to modulate their expression and to program Treg function. PMID: 21654845
the mode of the chromatin fiber compaction changes depending both on salt environment and linker histone H1 PMID: 16185066
Phosphorylation of human H1 variants occurs nonrandomly during both interphase and mitosis and distinct serine- or threonine-specific kinases are involved in different cell cycle phases. PMID: 16377619
Phosphorylation at threonine 10 appears in prometaphase and disappears in telophase, and that this hyperphosphorylated form of H1.5 is mainly chromatin-bound in metaphase when chromatin condensation is maximal. PMID: 19136008

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Subcellular Location

Nucleus. Chromosome. Note=According to PubMed:15911621 more commonly found in heterochromatin. According to PubMed:10997781 associates with actively transcribed chromatin and not heterochromatin.

Protein Families

Histone H1/H5 family

Tissue Specificity

Ubiquitous. Expressed in the majority of the cell lines tested and in testis.

Database Links

HGNC: 4719

OMIM: 142711

KEGG: hsa:3009

STRING: 9606.ENSP00000330074

UniGene: Hs.131956

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