Phospho-HIST1H1D (T179) Antibody

Immunofluorescence staining of Hela with CSB-PA010379OA179phHU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Chromatin Immunoprecipitation Hela (4*10⁶) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5μg anti-HIST1H1D (CSB-PA010379OA179phHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.