anti-Human Phospho-HIST1H1D (T179) Antibody raised in Rabbit

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Code	CSB-PA010379OA179phHU
Size	US$319
Uniprot No.	P16402
Abbreviation	Histone H1.3
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Image	Immunofluorescence staining of Hela with CSB-PA010379OA179phHU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Chromatin Immunoprecipitation Hela (4*10⁶) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5μg anti-HIST1H1D (CSB-PA010379OA179phHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
Immunogen	Peptide sequence around site of phospho-Thr (179) derived from human Histone H1.3.
Raised in	Rabbit
Species Reactivity	Human
Tested Applications	ELISA, IF, ChIP; Recommended dilution: IF:1:50-1:200
Relevance	Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Form	Liquid
Conjugate	Non-conjugated
Storage Buffer	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method	Antigen Affinity Purified
Isotype	IgG
Clonality	Polyclonal
Alias	Histone H1.3 (Histone H1c) (Histone H1s-2), HIST1H1D, H1F3
Immunogen Species	Human
Protocols	ELISA Protocol Immunofluorescence (IF) Protocol Native Chromatin Immunoprecipitation(ChIP) Protocol
Research Area	Epigenetics and Nuclear Signaling
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Function	Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Subcellular Location	Nucleus, Chromosome
Protein Families	Histone H1/H5 family
Database Links	HGNC: 4717 OMIM: 142210 KEGG: hsa:3007 STRING: 9606.ENSP00000244534 UniGene: Hs.136857