Phospho-HIST1H1D (T179) Antibody

^Datasheet

Specification
Target

Code	CSB-PA010379OA179phHU
Size	US$319
Uniprot No.	P16402
Abbreviation	Histone H1.3
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Image	Immunofluorescence staining of Hela with CSB-PA010379OA179phHU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). Chromatin Immunoprecipitation Hela(4*10⁶)were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H1D(CSB-PA010379OA179phHU)or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers against the β-Globin promoter.
Immunogen	Peptide sequence around site of phospho-Thr (179) derived from human Histone H1.3.
Raised in	Rabbit
Species Reactivity	Human
Tested Applications	ELISA, IF, ChIP; Recommended dilution: IF:1:50-1:200
Relevance	Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Form	Liquid
Conjugate	Non-conjugated
Storage Buffer	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method	Antigen Affinity Purified
Isotype	IgG
Clonality	Polyclonal
Alias	Histone H1.3 (Histone H1c) (Histone H1s-2), HIST1H1D, H1F3
Immunogen Species	Homo sapiens (Human)
Protocols	ELISA Protocol Immunofluorescence (IF) Protocol Native Chromatin Immunoprecipitation(ChIP) Protocol
Research Area	Epigenetics and Nuclear Signaling
Target Names	HIST1H1D
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Function	Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Subcellular location	Nucleus, Chromosome

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CSB-PA010379OA179nphHU	HIST1H1D (Ab-179) Antibody	Human	ELISA, IF, ChIP
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CSB-PA010379OA106bhbHU	β-hydroxybutyryl-HIST1H1D (K106) Antibody	Human	ELISA, WB, ICC
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HIST1H1D Antibodies for Human

Code	Product Name	Species Reactivity	Application
CSB-PA010379OA34nbhbHU	HIST1H1D (Ab-34) Antibody	Human	ELISA, IHC, IF
CSB-PA010379OA90nbhbHU	HIST1H1D (Ab-90) Antibody	Human	ELISA, IHC, IF
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HIST1H1D Proteins

HIST1H1D Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP010379HU	Recombinant Human Histone H1.3(HIST1H1D),Yeast	Yeast
CSB-EP010379HU	Recombinant Human Histone H1.3(HIST1H1D),Escherichia Coli	E.coli
CSB-BP010379HU	Recombinant Human Histone H1.3(HIST1H1D),Baculovirus	Baculovirus
CSB-MP010379HU	Recombinant Human Histone H1.3(HIST1H1D),Mammalian cell	Mammalian cell

HIST1H1D Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-YP010379MO	Recombinant Mouse Histone H1.3(Hist1h1d),Yeast	Yeast
CSB-EP010379MO	Recombinant Mouse Histone H1.3(Hist1h1d),Escherichia Coli	E.coli
CSB-BP010379MO	Recombinant Mouse Histone H1.3(Hist1h1d),Baculovirus	Baculovirus
CSB-MP010379MO	Recombinant Mouse Histone H1.3(Hist1h1d),Mammalian cell	Mammalian cell

HIST1H1D cDNA

HIST1H1D cDNA for Mus musculus (Mouse)

Code	Product Name	Vector	Accession NO.
CSB-CL010379MO	Hist1h1d	pUC	NM_145713.3

HIST1H1D ELISA Kit

HIST1H1D ELISA Kit for Homo sapiens (Human)

Code	Product Name	Sample Types	Sensitivity
CSB-EL010379HU	Human Histone H1.3(HIST1H1D) ELISA kit		6.25 pg/mL

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Phospho-HIST1H1D (T179) Antibody

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