Phospho-HIST1H1E (T17) Antibody

Datasheet
Code CSB-PA010380OA17phHU
Size US$319
Uniprot No. P10412
Image
  • Western Blot
    Detected samples: Jurkat whole cell lysate, Hela whole cell lysate; Untreated (-) or treated (+) with Calyculin A and EGF
    All lanes: HIST1H1E antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/40000 dilution
    Predicted band size: 22 kDa
    Observed band size: 22 kDa

  • Immunofluorescence staining of Hela cells with CSB-PA010380OA17phHU at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunogen Peptide sequence around site of Phospho-Thr (17) derived from Human Histone H1.4
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IF; Recommended dilution: WB:1:500-1:2000, IF:1:1-1:10
Relevance Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method Antigen Affinity Purified
Isotype IgG
Clonality Polyclonal
Alias Histone H1.4 (Histone H1b) (Histone H1s-4), HIST1H1E, H1F4
Immunogen Species Human
Research Area Epigenetics and Nuclear Signaling
Target Names HIST1H1E
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Involvement in disease Rahman syndrome (RMNS)
Subcellular Location Nucleus, Chromosome
Protein Families Histone H1/H5 family
Database Links

HGNC: 4718

OMIM: 142220

KEGG: hsa:3008

STRING: 9606.ENSP00000307705

UniGene: Hs.248133

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