Phospho-HIST1H3A (T3) Antibody

Datasheet
Code CSB-PA010418OA03phHU
Size US$319
Uniprot No. P68431
Image
  • Western Blot
    Detected samples: Jurkat whole cell lysate, Hela whole cell lysate; Untreated (-) or treated (+) with Calyculin A and EGF
    All lanes: HIST1H3A antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/40000 dilution
    Predicted band size: 16 kDa
    Observed band size: 16 kDa

  • Immunoprecipitating HIST1H3A in Hela whole cell lysate
    Lane 1: Rabbit control IgG instead of CSB-PA010418OA03phHU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-PA010418OA03phHU (5µg) + Hela whole cell lysate (500µg)
    Lane 3: Hela whole cell lysate (20µg)

  • Chromatin Immunoprecipitation Hela (4*106, treated with 100nM calyculin A for 60min) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H3A (CSB-PA010418OA03phHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunoprecipitation (IP) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Immunogen Peptide sequence around site of Phospho-Thr (3) derived from Human Histone H3.1
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IP, ChIP; Recommended dilution: WB:1:500-1:2000, IP:1:200-1:2000
Relevance Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method Antigen Affinity Purified
Isotype IgG
Clonality Polyclonal
Alias Histone H3.1 (Histone H3/a) (Histone H3/b) (Histone H3/c) (Histone H3/d) (Histone H3/f) (Histone H3/h) (Histone H3/i) (Histone H3/j) (Histone H3/k) (Histone H3/l), HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J, H3FA; H3FL; H3FC; H3FB; H3FD; H3FI; H3FH; H3FK; H3FF; H3FJ
Immunogen Species Human
Research Area Epigenetics and Nuclear Signaling
Target Names HIST1H3A
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Involvement in disease Glioma (GLM)
Subcellular Location Nucleus, Chromosome
Protein Families Histone H3 family
Database Links

HGNC: 4766

OMIM: 137800

KEGG: hsa:8350

STRING: 9606.ENSP00000444823

UniGene: Hs.132854

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