Phospho-SGK223 (Y413) Antibody

Code CSB-PA007578
Size US$100
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  • Western Blot analysis of A549 cells using Phospho-Pragmin (Y413) Polyclonal Antibody
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Product Details

Uniprot No.
Target Names
PRAG1
Alternative Names
D8Ertd82e antibody; DKFZp761P0423 antibody; DNA segment, Chr 8, ERATO Doi 82 antibody; EC 2.7.10.2 antibody; Homolog of rat pragma of Rnd2 antibody; LOC157285 antibody; PRAGMIN antibody; SG223_HUMAN antibody; Sgk223 antibody; Sugen kinase 223 antibody; Tyrosine protein kinase SgK223 antibody; Tyrosine-protein kinase SgK223 antibody
Raised in
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Synthesized peptide derived from Human Pragmin around the phosphorylation site of Y413.
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Isotype
IgG
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form
Liquid
Tested Applications
WB, IHC, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Catalytically inactive protein kinase that acts as a scaffold protein. Functions as an effector of the small GTPase RND2, which stimulates RhoA activity and inhibits NGF-induced neurite outgrowth. Promotes Src family kinase (SFK) signaling by regulating the subcellular localization of CSK, a negative regulator of these kinases, leading to the regulation of cell morphology and motility by a CSK-dependent mechanism. Acts as a critical coactivator of Notch signaling.
Gene References into Functions
  1. analysis of how the kinase fold mediates non-catalytic functions in SgK223 PMID: 29079850
  2. deregulation of the Pragmin-Csk axis may induce aberrant cell migration that contributes to tumor invasion and metastasis PMID: 27116701
  3. Results provide evidence that NACK was strongly expressed in a subset of non-small cell lung cancer (NSCLCL) samples and interference of NACK inhibits NSCLC progression through failing to activate Notch1 signaling complexes. PMID: 26782286
  4. Increased expression of SgK223 occurs in PDAC, and overexpression of SgK223 in pancreatic ductal epithelial cells promotes acquisition of a migratory and invasive phenotype through enhanced JAK1/Stat3 signaling PMID: 26215634
  5. Pragmin as a mammalian EPIYA effector and suggests that bacterial EPIYA effectors target Pragmin to subvert SFKs for successful infection. PMID: 21873224
  6. Rnd2 regulates neurite outgrowth by functioning as the RhoA activator through Pragmin, in contrast to Rnd1 and Rnd3 inhibiting RhoA signaling PMID: 16481321
  7. Also known as SgK223, a member of the NKF3 subfamily of eukaryotic protein kinases. PMID: 12471243

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Subcellular Location
Cytoplasm. Cell junction, focal adhesion. Nucleus.
Protein Families
Protein kinase superfamily
Database Links

HGNC: 25438

OMIM: 617344

KEGG: hsa:157285

STRING: 9606.ENSP00000330930

UniGene: Hs.583863

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