Recombinant Human Tyrosine-protein kinase SgK223 (SGK223), partial

Code CSB-YP801267HU
MSDS
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Source Yeast
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Code CSB-EP801267HU
MSDS
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Source E.coli
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Code CSB-EP801267HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP801267HU
MSDS
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Source Baculovirus
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Code CSB-MP801267HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PRAG1
Uniprot No.
Alternative Names
D8Ertd82e; DKFZp761P0423; DNA segment, Chr 8, ERATO Doi 82; EC 2.7.10.2; Homolog of rat pragma of Rnd2; LOC157285; PRAGMIN ; SG223_HUMAN; Sgk223; Sugen kinase 223; Tyrosine protein kinase SgK223; Tyrosine-protein kinase SgK223
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalytically inactive protein kinase that acts as a scaffold protein. Functions as an effector of the small GTPase RND2, which stimulates RhoA activity and inhibits NGF-induced neurite outgrowth. Promotes Src family kinase (SFK) signaling by regulating the subcellular localization of CSK, a negative regulator of these kinases, leading to the regulation of cell morphology and motility by a CSK-dependent mechanism. Acts as a critical coactivator of Notch signaling.
Gene References into Functions
  1. analysis of how the kinase fold mediates non-catalytic functions in SgK223 PMID: 29079850
  2. deregulation of the Pragmin-Csk axis may induce aberrant cell migration that contributes to tumor invasion and metastasis PMID: 27116701
  3. Results provide evidence that NACK was strongly expressed in a subset of non-small cell lung cancer (NSCLCL) samples and interference of NACK inhibits NSCLC progression through failing to activate Notch1 signaling complexes. PMID: 26782286
  4. Increased expression of SgK223 occurs in PDAC, and overexpression of SgK223 in pancreatic ductal epithelial cells promotes acquisition of a migratory and invasive phenotype through enhanced JAK1/Stat3 signaling PMID: 26215634
  5. Pragmin as a mammalian EPIYA effector and suggests that bacterial EPIYA effectors target Pragmin to subvert SFKs for successful infection. PMID: 21873224
  6. Rnd2 regulates neurite outgrowth by functioning as the RhoA activator through Pragmin, in contrast to Rnd1 and Rnd3 inhibiting RhoA signaling PMID: 16481321
  7. Also known as SgK223, a member of the NKF3 subfamily of eukaryotic protein kinases. PMID: 12471243

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Subcellular Location
Cytoplasm. Cell junction, focal adhesion. Nucleus.
Protein Families
Protein kinase superfamily
Database Links

HGNC: 25438

OMIM: 617344

KEGG: hsa:157285

STRING: 9606.ENSP00000330930

UniGene: Hs.583863

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