Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) RAPGEF2 Polyclonal antibody

Uniprot No.

Q9Y4G8

Target Names

RAPGEF2

Alternative Names

CNrasGEF antibody; Cyclic nucleotide ras GEF antibody; KIAA0313 antibody; Neural RAP guanine nucleotide exchange protein antibody; nRap GEP antibody; NRAPGEP antibody; PDZ domain containing guanine nucleotide exchange factor 1 antibody; PDZ domain-containing guanine nucleotide exchange factor 1 antibody; PDZ GEF1 antibody; PDZ-GEF1 antibody; PDZGEF1 antibody; RA GEF antibody; RA-GEF-1 antibody; Rap guanine nucleotide exchange factor 2 antibody; Rapgef2 antibody; Ras/Rap1-associating GEF-1 antibody; RPGF2_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Rap guanine nucleotide exchange factor 2 protein (1393-1498AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

FITC

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Background

Function

Functions as a guanine nucleotide exchange factor (GEF), which activates Rap and Ras family of small GTPases by exchanging bound GDP for free GTP in a cAMP-dependent manner. Serves as a link between cell surface receptors and Rap/Ras GTPases in intracellular signaling cascades. Acts also as an effector for Rap1 by direct association with Rap1-GTP thereby leading to the amplification of Rap1-mediated signaling. Shows weak activity on HRAS. It is controversial whether RAPGEF2 binds cAMP and cGMP or not. Its binding to ligand-activated beta-1 adrenergic receptor ADRB1 leads to the Ras activation through the G(s)-alpha signaling pathway. Involved in the cAMP-induced Ras and Erk1/2 signaling pathway that leads to sustained inhibition of long term melanogenesis by reducing dendrite extension and melanin synthesis. Provides also inhibitory signals for cell proliferation of melanoma cells and promotes their apoptosis in a cAMP-independent nanner. Regulates cAMP-induced neuritogenesis by mediating the Rap1/B-Raf/ERK signaling through a pathway that is independent on both PKA and RAPGEF3/RAPGEF4. Involved in neuron migration and in the formation of the major forebrain fiber connections forming the corpus callosum, the anterior commissure and the hippocampal commissure during brain development. Involved in neuronal growth factor (NGF)-induced sustained activation of Rap1 at late endosomes and in brain-derived neurotrophic factor (BDNF)-induced axon outgrowth of hippocampal neurons. Plays a role in the regulation of embryonic blood vessel formation and in the establishment of basal junction integrity and endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR and cadherin CDH5 expression at allantois endothelial cell-cell junctions.

Gene References into Functions

Cdk5-mediated phosphorylation of RapGEF2 controls neuronal migration in the developing cerebral cortex. PMID: 25189171
local accumulation of PDZGEF at the apical membrane during establishment of epithelial polarity is mediated by electrostatic interactions between positively charged side chains in the PDZ domain and negatively charged phosphatidic acid. PMID: 24858808
SCF(FBXW11) bound, polyubiquitylated, and destabilized RAPGEF2, a guanine nucleotide exchange factor that activates the small GTPase RAP1 PMID: 25332235
NEDD4-1 regulates cell migration and invasion through ubiquitination of CNrasGEF in vitro. PMID: 24340059
We report that in response to factors that promote cell motility, the Rap guanine exchange factor RAPGEF2 is rapidly phosphorylated by I-kappa-B-kinase-beta and casein kinase-1alpha and consequently degraded by the proteasome. PMID: 24290981
Data indicate that expression of Rapgef2 in embryonic kidney 293T cells enables cAMP-Rap1-ERK signaling. PMID: 23800469
DNA methylation shows genome-wide association of NFIX, RAPGEF2 and MSRB3 with gestational age at birth. PMID: 22422452
PDZ-GEF activates Rap1 under resting conditions to stabilize cell-cell junctions and maintain basal integrity PMID: 21840392
Regulation of G protein-linked guanine nucleotide exchange factors for Rho, PDZ-RhoGEF, and LARG by tyrosine phosphorylation: evidence of a role for focal adhesion kinase PMID: 11799111
PSD-95/Dlg/ZO-1 homology (PDZ)-RhoGEF was isolated from mouse brain as a plexin-B1-specific interacting protein PMID: 12196628
Data show that the interaction of PDZ-GEF1 with an internalized neurotrophin receptor transported to late endosomes induces sustained activation of both Rap1 and ERK and neurite outgrowth. PMID: 17724123

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Subcellular Location

Cytoplasm. Cytoplasm, perinuclear region. Cell membrane. Late endosome. Cell junction.

Protein Families

RAPGEF2 family

Tissue Specificity

Expressed in primary neuronal and endocrine cells (at protein level). Highest expression levels in brain. Lower expression levels in heart, kidney, lung, placenta and blood leukocytes.

Database Links

HGNC: 16854

OMIM: 609530

KEGG: hsa:9693

STRING: 9606.ENSP00000264431

UniGene: Hs.744884