RHBDD3 Antibody

Code CSB-PA27742A0Rb
Size US$166
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Image
  • Western Blot
    Positive WB detected in: PC-3 whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate
    All lanes: RHBDD3 antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 41 kDa
    Observed band size: 41 kDa

  • IHC image of CSB-PA27742A0Rb diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of U251 cells with CSB-PA27742A0Rb at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) RHBDD3 Polyclonal antibody
Uniprot No.
Target Names
RHBDD3
Alternative Names
C22orf3 antibody; HS984G1A antibody; Pituitary tumor apoptosis antibody; PTAG antibody; RHBD3_HUMAN antibody; RHBDD3 antibody; Rhomboid domain containing 3 antibody; Rhomboid domain-containing protein 3 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Peptide sequence from Human Rhomboid domain-containing protein 3 protein (304-323AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The RHBDD3 Antibody (Product code: CSB-PA27742A0Rb) is Non-conjugated. For RHBDD3 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA27742B0Rb RHBDD3 Antibody, HRP conjugated ELISA
FITC CSB-PA27742C0Rb RHBDD3 Antibody, FITC conjugated
Biotin CSB-PA27742D0Rb RHBDD3 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Gene References into Functions
  1. RHBDD3 is a target gene of the BACH1 transcription factor according to ChIP-seq analysis in HEK 293 cells. PMID: 21555518
  2. isolation of a novel differentially methylated chromosome 22 CpG island-associated gene PTAG PMID: 15105437
  3. Loss of PTAG contributes to a blunted apoptotic response and is likely to predispose cells toward malignant transformation and resistance to chemotherapeutic interventions. PMID: 17117413
  4. the EWSR1 promoter functions in a bidirectional manner, thereby regulating also RHBDD3 PMID: 19212622
Subcellular Location
Membrane; Multi-pass membrane protein.
Database Links

HGNC: 1308

KEGG: hsa:25807

STRING: 9606.ENSP00000216085

UniGene: Hs.106730

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