RHBDD3 Antibody

Datasheet

Code	CSB-PA27742A0Rb
Size	US$166
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Image	Western Blot Positive WB detected in: PC-3 whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate All lanes: RHBDD3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 41 kDa Observed band size: 41 kDa IHC image of CSB-PA27742A0Rb diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of U251 cells with CSB-PA27742A0Rb at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) RHBDD3 Polyclonal antibody

Uniprot No.

Q9Y3P4

Target Names

RHBDD3

Alternative Names

C22orf3 antibody; HS984G1A antibody; Pituitary tumor apoptosis antibody; PTAG antibody; RHBD3_HUMAN antibody; RHBDD3 antibody; Rhomboid domain containing 3 antibody; Rhomboid domain-containing protein 3 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Peptide sequence from Human Rhomboid domain-containing protein 3 protein (304-323AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The RHBDD3 Antibody (Product code: CSB-PA27742A0Rb) is Non-conjugated. For RHBDD3 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA27742B0Rb	RHBDD3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA27742C0Rb	RHBDD3 Antibody, FITC conjugated
Biotin	CSB-PA27742D0Rb	RHBDD3 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Gene References into Functions

RHBDD3 is a target gene of the BACH1 transcription factor according to ChIP-seq analysis in HEK 293 cells. PMID: 21555518
isolation of a novel differentially methylated chromosome 22 CpG island-associated gene PTAG PMID: 15105437
Loss of PTAG contributes to a blunted apoptotic response and is likely to predispose cells toward malignant transformation and resistance to chemotherapeutic interventions. PMID: 17117413
the EWSR1 promoter functions in a bidirectional manner, thereby regulating also RHBDD3 PMID: 19212622

Subcellular Location

Membrane; Multi-pass membrane protein.

Database Links

HGNC: 1308

KEGG: hsa:25807

STRING: 9606.ENSP00000216085

UniGene: Hs.106730

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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