Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) RHOH Polyclonal antibody

Uniprot No.

Q15669

Target Names

RHOH

Alternative Names

ARHH antibody; GTP binding protein TTF antibody; GTP-binding protein TTF antibody; OTTHUMP00000125213 antibody; Ras homolog gene family member H antibody; Rho related GTP binding protein antibody; Rho related GTP binding protein RhoH antibody; Rho-related GTP-binding protein RhoH antibody; RHOH antibody; RHOH_HUMAN antibody; Translocation three four protein antibody; TTF antibody; TTF translocation three four antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Rho-related GTP-binding protein RhoH protein (1-188AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

FITC

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Background

Function

Negative regulator of hematopoietic progenitor cell proliferation, survival and migration. Critical regulator of thymocyte development and T-cell antigen receptor (TCR) signaling by mediating recruitment and activation of ZAP70. Required for phosphorylation of CD3Z, membrane translocation of ZAP70 and subsequent activation of the ZAP70-mediated pathways. Essential for efficient beta-selection and positive selection by promoting the ZAP70-dependent phosphorylation of the LAT signalosome during pre-TCR and TCR signaling. Crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Plays critical roles in mast cell function by facilitating phosphorylation of SYK in Fc epsilon RI-mediated signal transduction. Essential for the phosphorylation of LAT, LCP2, PLCG1 and PLCG2 and for Ca(2+) mobilization in mast cells. Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins. Inhibits the activation of NF-kappa-B by TNF and IKKB and the activation of CRK/p38 by TNF. Inhibits activities of RAC1, RHOA and CDC42. Negatively regulates leukotriene production in neutrophils.

Gene References into Functions

extracellular microenvironment signals regulate RhoH and Kaiso to modulate actin-cytoskeleton structure and transcriptional activity during T cell migration PMID: 27574848
These results highlight a novel negative regulatory role for RhoH in eosinophil differentiation, most likely in consequence of altered GATA-2 levels. PMID: 27740624
Mutations in hematopoiesis-specific Rho GTPases Rac2 and RhoH lead to a wide range of human blood disorders. (Review) PMID: 23850828
Our current results indicate differential requirements for RhoH in the development of TCRalphabeta CD8alphaalpha IELs compared to other subsets of T cells including agonist selected T cells. PMID: 23499578
RHOH deficiency leads to T cell defects and persistent epidermodysplasia verruciformis-HPV infections. PMID: 22850876
conclude that RhoH expression provides a key molecular determinant that allows T cells to switch between sensing chemokine-mediated go signals and TCR-dependent stop signals PMID: 22689994
RhoH plays a critical role in prosurvival chronic lymphocytic leukemia cell-cell and cell-microenvironment interactions with this agent. PMID: 22474251
Repression of the RHOH gene by JunD PMID: 21473742
RhoH is a key adapter protein that maintains LCK in the inactive state, contributing to the regulation of both pre-TCR and TCR signalling during T-cell development. PMID: 20851766
propose that RhoH functions as a negative regulator for IL3-induced signals through modulation of the JAK-STAT pathway PMID: 20738848
Data suggest that RhoH function might be regulated by lysosomal degradation of RhoH protein following TCR complex but not BCR activation. PMID: 19950172
RhoH has a function in the progression of B-cell chronic lymphocytic leukemia (CLL) in a murine model and show RhoH expression is altered in human primary lymphocytic (CLL) samples. PMID: 19847197
5' end heterogeneity of RhoH mRNAs was observed, due to alternative splicing of some 5' exons and to the use of these different transcription start sites. PMID: 15563831
In both Richter's transformation and prolymphocytic transformation, high-levels of AID mRNA expression and high-frequency mutations of RhoH protein genes were detected. PMID: 16541139
Aberrant somatic hypermutation of RhoH gene is associated with diffuse large B cell lymphoma PMID: 17443219
RHOH mutation is associated with splenic and nodal marginal zone B-cell lymphomas PMID: 17476282
Underexpression of RhoH is associated with Hairy Cell Leukemia PMID: 18559497
low expression of the RhoH transcript was a predictor of worse prognosis in both overall and disease-free survival. PMID: 18691253
RhoH/TTF represents an inducible feedback inhibitor in neutrophils that is involved in the limitation of innate immune responses. PMID: 19414807

Hide All

Involvement in disease

A chromosomal aberration involving RHOH is found in a non-Hodgkin lymphoma cell line. Translocation t(3;4)(q27;p11) with BCL6.

Subcellular Location

Cytoplasm. Cell membrane; Lipid-anchor; Cytoplasmic side.

Protein Families

Small GTPase superfamily, Rho family

Tissue Specificity

Expressed only in hematopoietic cells. Present at very high levels in the thymus, less abundant in the spleen, and least abundant in the bone marrow. Expressed at a higher level in the TH1 subtype of T-helper cells than in the TH2 subpopulation. Expressed

Database Links

HGNC: 686

OMIM: 602037

KEGG: hsa:399

STRING: 9606.ENSP00000371219

UniGene: Hs.654594