RHOT2 Antibody

Datasheet

Code	CSB-PA810289LA01HU
Size	US$166
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Image	IHC image of CSB-PA810289LA01HU diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA810289LA01HU diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) RHOT2 Polyclonal antibody

Uniprot No.

Q8IXI1

Target Names

RHOT2

Alternative Names

ARHT2 antibody; C16orf39 antibody; hMiro 2 antibody; hMiro-2 antibody; hMiro2 antibody; MIRO-2 antibody; MIRO2_HUMAN antibody; Mitochondrial Rho GTPase 2 antibody; Ras homolog gene family member T2 antibody; RASL antibody; RHOT2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Mitochondrial Rho GTPase 2 protein (84-300AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The RHOT2 Antibody (Product code: CSB-PA810289LA01HU) is Non-conjugated. For RHOT2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA810289LB01HU	RHOT2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA810289LC01HU	RHOT2 Antibody, FITC conjugated
Biotin	CSB-PA810289LD01HU	RHOT2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.

Gene References into Functions

The Miro2-mediated mitochondrial transport in neurons and recently highlighted involvement of Miro2 proteins in mitochondrial turnover, emerging as a key process affected in neurodegeneration. PMID: 24256248
Miro proteins serve as a [Ca(2+)](c)-sensitive switch and bifunctional regulator for both the motility and fusion-fission dynamics of the mitochondria. PMID: 19098100

Subcellular Location

Mitochondrion outer membrane; Single-pass type IV membrane protein. Note=Colocalizes with MGARP and RHOT2 at the mitochondria.

Protein Families

Mitochondrial Rho GTPase family

Tissue Specificity

Ubiquitously expressed. Highly expressed in heart, liver, skeletal muscle, kidney and pancreas.

Database Links

HGNC: 21169

OMIM: 613889

KEGG: hsa:89941

STRING: 9606.ENSP00000321971

UniGene: Hs.513242

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Protein Service

Gene Synthesis Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Mitophagy

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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