RIN1 Antibody

Datasheet

Code	CSB-PA019726LA01HU
Size	US$166
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Image	Immunofluorescence staining of Hela cells with CSB-PA019726LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) RIN1 Polyclonal antibody

Uniprot No.

Q13671

Target Names

RIN1

Alternative Names

RIN1 antibody; Ras and Rab interactor 1 antibody; Ras inhibitor JC99 antibody; Ras interaction/interference protein 1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Ras and Rab interactor 1 protein (192-341AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The RIN1 Antibody (Product code: CSB-PA019726LA01HU) is Non-conjugated. For RIN1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA019726LB01HU	RIN1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA019726LC01HU	RIN1 Antibody, FITC conjugated
Biotin	CSB-PA019726LD01HU	RIN1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IF

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200

Protocols

ELISA Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Ras effector protein, which may serve as an inhibitory modulator of neuronal plasticity in aversive memory formation. Can affect Ras signaling at different levels. First, by competing with RAF1 protein for binding to activated Ras. Second, by enhancing signaling from ABL1 and ABL2, which regulate cytoskeletal remodeling. Third, by activating RAB5A, possibly by functioning as a guanine nucleotide exchange factor (GEF) for RAB5A, by exchanging bound GDP for free GTP, and facilitating Ras-activated receptor endocytosis.

Gene References into Functions

Findings suggest that RIN1 plays an important oncogenic role in clear cell renal cell carcinoma malignancy by activation of EGFR signaling through interacting with Rab25. PMID: 28612496
Smad2 is a key scaffold, allowing RIN1 to act as a GTP exchange factor for MFN2-GTPase activation to promote mitochondrial ATP synthesis and suppress superoxide production during mitochondrial fusion. PMID: 27184078
Overexpression experiments in tandem with pull-down assays indicated that specific Ser291/292 phosphorylation of RIN1 favored binding to activated Ras PMID: 27137893
analysis of a novel deregulated mechanism in chronic myeloid leukemia patients, indicating BIN1 and RIN1 as players in the maintenance of the abnormal RTK signaling in this hematological disease PMID: 26194865
Findings suggest that RIN1 orchestrates RAB5 activation, ABL kinase activation and BIN1 recruitment to determine EGFR fate. PMID: 22976291
over-expression of RIN1 may play an important role in the progression of non-small cell lung cancer and RIN1 expression may offer a valuable marker for predicting the outcome of patients with NSCLC PMID: 21925717
RIN1 plays an important role in gastric adenocarcinoma progression and that a high RIN1 expression predicts an unfavorable prognosis in gastric adenocarcinoma patients. PMID: 22562267
RIN1 expression could be a potential prognostic predictor for the melanoma patients and provide a potential target therapy for melanoma treatment. PMID: 22627834
RIN1 expression could be a potential prognostic predictor for bladder urothelial carcinoma (UC) patients. PMID: 22249975
These data identify a novel PKD signaling pathway through RIN1 and Abl kinases that is involved in the regulation of actin remodeling and cell migration. PMID: 21209314
BCR-ABL1 kinase activity is regulated by RIN1 PMID: 21102429
Plays an important role in both insulin receptor membrane trafficking and signaling. PMID: 16457816
These results indicate that the RIN1 gene serves as an important signal transduction system for evaluating the malignancy of colorectal cancer. PMID: 17390061
Rin1 regulates EGFR degradation in cooperation with STAM PMID: 17403676
In a tumor cell line, RIN1 silencing may contribute to breast cancer progression. PMID: 18089779
Specific residues of RIN1 are required for its interaction with Rab5, binding to the endosomal membranes and subsequent regulation of the fusion reaction. PMID: 19032933
Mutations in the Vps9 domain of Rin1 lead to a loss-of-function phenotype, indicating a specific structure-function relationship between Rab5 and Rin1. PMID: 19118546
Data demonstrate that proper internalization and endocytic trafficking are critical for EGFR-mediated signaling in A549 cells and suggest that up-regulation of Rin1 in A549 cell lines may contribute to their proliferative nature. PMID: 19570984
the novel RIN1 mRNA was found to be expressed in gastric and colon cancer cell lines, suggesting that it is an important gene for the function of cancer cells. PMID: 19806790

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Subcellular Location

Cytoplasm. Membrane. Cytoplasm, cytoskeleton. Note=Some amount is membrane-associated.

Protein Families

RIN (Ras interaction/interference) family

Tissue Specificity

Expressed in all tissues examined with high levels in brain, placenta and pancreas.

Database Links

HGNC: 18749

OMIM: 605965

KEGG: hsa:9610

STRING: 9606.ENSP00000310406

UniGene: Hs.1030

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Related pathways

Ras signaling pathway

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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