PHF7 Antibody

Datasheet
Code CSB-PA017918LA01HU
See More Details Free Antibody trial simple
Size US$299
Uniprot No. Q9BWX1
Image
  • IHC image of CSB-PA017918LA01HU diluted at 1:300 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA017918LA01HU diluted at 1:300 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunogen Recombinant Human PHD finger protein 7 protein (277-368AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, IHC; Recommended dilution: IHC:1:200-1:500
Relevance May play a role in spermatogenesis.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias PHD finger protein 7 (Testis development protein NYD-SP6), PHF7
Immunogen Species Human
Research Area Signal Transduction
Target Names PHF7
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function May play a role in spermatogenesis.
Subcellular Location Nucleus
Tissue Specificity Highly expressed in Sertoli cells, but not in germ cells in adult testis. Expression in embryonic testis is 30-times lower. Highly expressed in colon, spleen, white blood cells, pancreas, lung, liver, placenta and brain. Detected at lower levels in thymus
Database Links

HGNC: 18458

KEGG: hsa:51533

STRING: 9606.ENSP00000333024

UniGene: Hs.372719

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