SEC14L2 Antibody

Datasheet

Code	CSB-PA020937LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: PC-3 whole cell lysate All lanes: SEC14L2 antibody at 4.28µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47, 45, 37 kDa Observed band size: 47 kDa IHC image of CSB-PA020937LA01HU diluted at 1:400 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA020937LA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA020937LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) SEC14L2 Polyclonal antibody

Uniprot No.

O76054

Target Names

SEC14L2

Alternative Names

Alpha tocopherol associated protein antibody; Alpha-tocopherol-associated protein antibody; C22orf6 antibody; hTAP antibody; KIAA1186 antibody; KIAA1658 antibody; MGC65053 antibody; OTTHUMP00000198972 antibody; OTTHUMP00000198973 antibody; OTTHUMP00000198974 antibody; OTTHUMP00000199021 antibody; S14L2_HUMAN antibody; SEC14 (S. cerevisiae) like 2 antibody; SEC14 like 2 (S. cerevisiae) antibody; SEC14 like protein 2 antibody; SEC14-like protein 2 antibody; Sec14l2 antibody; SPF antibody; Squalene transfer protein antibody; Supernatant protein factor antibody; TAP antibody; TAP1 antibody; tocopherol-associated protein antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human SEC14-like protein 2 protein (233-358AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The SEC14L2 Antibody (Product code: CSB-PA020937LA01HU) is Non-conjugated. For SEC14L2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA020937LB01HU	SEC14L2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA020937LC01HU	SEC14L2 Antibody, FITC conjugated
Biotin	CSB-PA020937LD01HU	SEC14L2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Carrier protein. Binds to some hydrophobic molecules and promotes their transfer between the different cellular sites. Binds with high affinity to alpha-tocopherol. Also binds with a weaker affinity to other tocopherols and to tocotrienols. May have a transcriptional activatory activity via its association with alpha-tocopherol. Probably recognizes and binds some squalene structure, suggesting that it may regulate cholesterol biosynthesis by increasing the transfer of squalene to a metabolic active pool in the cell.

Gene References into Functions

Here, the authors report that the zebrafish/human phosphatidylinositol transfer protein Sec14l3/SEC14L2 act as GTPase proteins to transduce Wnt signals from Frizzled to phospholipase C (PLC). PMID: 28463110
hTAP1 reduces the in vitro activity of the phosphatidylinositol-3-kinase gamma (PI3Kgamma) indicating the formation of a stalled/inactive hTAP1/PI3Kgamma heterodimer. PMID: 24983950
PI3Kgamma-induced VEGF expression was reduced when the human tocopherol-associated protein 1 (hTAP1/SEC14L2) was overexpressed suggesting formation of an inactive PI3Kgamma/hTAP1 heterodimer. PMID: 25290554
SEC14L2 enabled RNA replication of diverse HCV genotypes in several human hepatoma cell lines PMID: 26266980
novel association between the rs737723 polymorphism (SEC14L2/TAP) and higher primary open-angle glaucoma risk PMID: 23401652
TAP expression was significantly correlated with Her2/neu receptor expression and breast cancer stage. PMID: 23411208
Discusses cloning of rat SEC14-like protein 3 and mentions three human SPF-related genes, known as SEC14-like protein 1, 2 and 3. PMID: 15033454
SPF has two domain topology, and the ligand binding cavity has a peculiar horseshoe-like shape PMID: 12429094
phosphorylation enhances its ability to stimulate microsomal squalene monooxygenase; phosphorylation may provide a means for the rapid short term modulation of cholesterol synthesis PMID: 12454003
Data report the three-dimensional crystal structure of human supernatant protein factor in complex with RRR-alpha-tocopherylquinone, the major physiological oxidation product of RRR-alpha-tocopherol, at a resolution of 1.95A. PMID: 12972248
TAP not only mediates vitamin E absorption to facilitate vitamin E antiproliferation effect in prostate cancer cells, but also functions like a tumor suppressor gene to control cancer cell viability through a non-vitamin E manner. PMID: 16267002
reduced expression of TAP was associated with the cell proliferation status of prostate cancer, adverse pathological parameters and the increased risk of recurrence PMID: 17334589
Genetic variation in TTPA and SEC14L2 is associated with serum alpha-tocopherol but does not have a direct effect on prostate cancer when vitamin E is administered. PMID: 19190344
These findings are consistent with alpha-tocopherol-associated protein acting as an antiproliferative factor in estrogen-receptor-positive luminal cells in normal/benign breast tissue PMID: 19305383
Fndings raise the possibility that TAP/Sec14L2 may serve as a tumor suppressor in breast carcinogenesis. PMID: 19909011

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Subcellular Location

Cytoplasm. Nucleus. Note=Cytoplasmic in absence of alpha-tocopherol, and nuclear in presence of alpha-tocopherol.

Tissue Specificity

Widely expressed. Strong expression in liver, brain and prostate.

Database Links

HGNC: 10699

OMIM: 607558

KEGG: hsa:23541

STRING: 9606.ENSP00000316203

UniGene: Hs.335614

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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