SEC14L2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA599766A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Hela whole cell lysate(30µg), HepG2 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), U-251MG whole cell lysate(30µg), A549 whole cell lysate(30µg) All lanes: SEC14L2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 46 kDa Observed band size: 40 kDa Exposure time：1min Overlay Peak curve showing BxPC-3 cells stained with CSB-RA599766A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

O76054

Target Names

SEC14L2

Alternative Names

Alpha tocopherol associated protein antibody; Alpha-tocopherol-associated protein antibody; C22orf6 antibody; hTAP antibody; KIAA1186 antibody; KIAA1658 antibody; MGC65053 antibody; OTTHUMP00000198972 antibody; OTTHUMP00000198973 antibody; OTTHUMP00000198974 antibody; OTTHUMP00000199021 antibody; S14L2_HUMAN antibody; SEC14 (S. cerevisiae) like 2 antibody; SEC14 like 2 (S. cerevisiae) antibody; SEC14 like protein 2 antibody; SEC14-like protein 2 antibody; Sec14l2 antibody; SPF antibody; Squalene transfer protein antibody; Supernatant protein factor antibody; TAP antibody; TAP1 antibody; tocopherol-associated protein antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human SEC14L2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

14C3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SEC14L2, also known as alpha-tocopherol-associated protein or supernatant protein factor, plays a crucial role in lipid metabolism and vitamin E transport within cells. This protein facilitates the intracellular trafficking of alpha-tocopherol and participates in cholesterol biosynthesis by functioning as a squalene transfer protein. Its involvement in these fundamental metabolic pathways makes SEC14L2 a valuable target for researchers investigating lipid homeostasis, vitamin E biology, and related signal transduction mechanisms.

This recombinant monoclonal antibody, clone 14C3, offers the reproducibility and consistency that demanding research applications require. Produced using recombinant technology with a defined sequence, this rabbit IgG antibody eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived antibodies, ensuring your experimental results remain comparable across studies and over time.

Validated for western blotting, flow cytometry, and ELISA, this antibody provides flexibility across multiple experimental workflows. Western blot analysis has confirmed reliable SEC14L2 detection in human cell lines including HeLa, HepG2, HEK293, U-251MG, and A549, demonstrating consistent performance across diverse cellular contexts. The observed band appears at approximately 40 kDa, slightly below the predicted 46 kDa molecular weight, a difference likely attributable to post-translational processing or protein cleavage events. Flow cytometry validation using BxPC-3 cells further confirms the antibody's utility for intracellular protein detection in fixed and permeabilized samples.

Supplied in a stabilized liquid format with glycerol for long-term storage at -20°C or -80°C, this affinity-purified antibody is ready for immediate use in your signal transduction research, lipid metabolism studies, or investigations into vitamin E-associated cellular processes.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Carrier protein. Binds to some hydrophobic molecules and promotes their transfer between the different cellular sites. Binds with high affinity to alpha-tocopherol. Also binds with a weaker affinity to other tocopherols and to tocotrienols. May have a transcriptional activatory activity via its association with alpha-tocopherol. Probably recognizes and binds some squalene structure, suggesting that it may regulate cholesterol biosynthesis by increasing the transfer of squalene to a metabolic active pool in the cell.

Gene References into Functions

Here, the authors report that the zebrafish/human phosphatidylinositol transfer protein Sec14l3/SEC14L2 act as GTPase proteins to transduce Wnt signals from Frizzled to phospholipase C (PLC). PMID: 28463110
hTAP1 reduces the in vitro activity of the phosphatidylinositol-3-kinase gamma (PI3Kgamma) indicating the formation of a stalled/inactive hTAP1/PI3Kgamma heterodimer. PMID: 24983950
PI3Kgamma-induced VEGF expression was reduced when the human tocopherol-associated protein 1 (hTAP1/SEC14L2) was overexpressed suggesting formation of an inactive PI3Kgamma/hTAP1 heterodimer. PMID: 25290554
SEC14L2 enabled RNA replication of diverse HCV genotypes in several human hepatoma cell lines PMID: 26266980
novel association between the rs737723 polymorphism (SEC14L2/TAP) and higher primary open-angle glaucoma risk PMID: 23401652
TAP expression was significantly correlated with Her2/neu receptor expression and breast cancer stage. PMID: 23411208
Discusses cloning of rat SEC14-like protein 3 and mentions three human SPF-related genes, known as SEC14-like protein 1, 2 and 3. PMID: 15033454
SPF has two domain topology, and the ligand binding cavity has a peculiar horseshoe-like shape PMID: 12429094
phosphorylation enhances its ability to stimulate microsomal squalene monooxygenase; phosphorylation may provide a means for the rapid short term modulation of cholesterol synthesis PMID: 12454003
Data report the three-dimensional crystal structure of human supernatant protein factor in complex with RRR-alpha-tocopherylquinone, the major physiological oxidation product of RRR-alpha-tocopherol, at a resolution of 1.95A. PMID: 12972248
TAP not only mediates vitamin E absorption to facilitate vitamin E antiproliferation effect in prostate cancer cells, but also functions like a tumor suppressor gene to control cancer cell viability through a non-vitamin E manner. PMID: 16267002
reduced expression of TAP was associated with the cell proliferation status of prostate cancer, adverse pathological parameters and the increased risk of recurrence PMID: 17334589
Genetic variation in TTPA and SEC14L2 is associated with serum alpha-tocopherol but does not have a direct effect on prostate cancer when vitamin E is administered. PMID: 19190344
These findings are consistent with alpha-tocopherol-associated protein acting as an antiproliferative factor in estrogen-receptor-positive luminal cells in normal/benign breast tissue PMID: 19305383
Fndings raise the possibility that TAP/Sec14L2 may serve as a tumor suppressor in breast carcinogenesis. PMID: 19909011

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Subcellular Location

Cytoplasm. Nucleus. Note=Cytoplasmic in absence of alpha-tocopherol, and nuclear in presence of alpha-tocopherol.

Tissue Specificity

Widely expressed. Strong expression in liver, brain and prostate.

Database Links

HGNC: 10699

OMIM: 607558

KEGG: hsa:23541

STRING: 9606.ENSP00000316203

UniGene: Hs.335614

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