SEMA4B Antibody

Datasheet

Code	CSB-PA868280LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: BxPC-3 whole cell lysate(30µg), HT29 whole cell lysate(30µg), U87 whole cell lysate(30µg) All lanes: SEMA4B antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 93,82 kDa Observed band size: 93 kDa Exposure time: 120s IHC image of CSB-PA868280LA01HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) SEMA4B Polyclonal antibody

Uniprot No.

Q9NPR2

Target Names

SEMA4B

Alternative Names

SEMA4B antibody; KIAA1745 antibody; SEMAC antibody; UNQ749/PRO1480 antibody; Semaphorin-4B antibody; Semaphorin-C antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Synthesized peptide derived from part region of human protein

Immunogen Species

Homo sapiens (Human)

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.

Gene References into Functions

SEMA4B may induce FoxO1 nuclear retention through suppressing PI3K/Akt signaling pathway, which subsequently inhibited cell growth through the direct nuclear target of FoxO1, p21. PMID: 25746385
Decrease in SEMA4b levels are associated with metastasis of non-small cell lung cancer. PMID: 25095981
showed that HIF-1alpha recognized a hypoxia-responsive element (HRE) of SEMA4B gene, which is required for HIF-1-repressed SEMA4B expression PMID: 24474252

Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Families

Semaphorin family

Database Links

HGNC: 10730

KEGG: hsa:10509

STRING: 9606.ENSP00000332204

UniGene: Hs.474935

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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