SHISA5 Antibody

Code CSB-PA818677LA01HU
Size US$166
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Image
  • Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA818677LA01HU at dilution of 1:100

  • Immunofluorescence staining of A549 cells with CSB-PA818677LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunoprecipitating SHISA5 in HepG2 whole cell lysate
    Lane 1: Rabbit control IgG (1µg) instead of CSB-PA818677LA01HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-PA818677LA01HU (6µg) + HepG2 whole cell lysate (500µg)
    Lane 3: HepG2 whole cell lysate (10µg)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) SHISA5 Polyclonal antibody
Uniprot No.
Target Names
SHISA5
Alternative Names
SHISA5 antibody; SCOTIN antibody; PSEC0133 antibody; Protein shisa-5 antibody; Putative NF-kappa-B-activating protein 120 antibody; Scotin antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Protein shisa-5 protein (56-137AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The SHISA5 Antibody (Product code: CSB-PA818677LA01HU) is Non-conjugated. For SHISA5 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA818677LB01HU SHISA5 Antibody, HRP conjugated ELISA
FITC CSB-PA818677LC01HU SHISA5 Antibody, FITC conjugated
Biotin CSB-PA818677LD01HU SHISA5 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, IHC, IF, IP
Recommended Dilution
Application Recommended Dilution
IHC 1:20-1:200
IF 1:50-1:500
IP 1:200-1:2000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Can induce apoptosis in a caspase-dependent manner and plays a role in p53/TP53-dependent apoptosis.
Gene References into Functions
  1. IFN-beta-induced SCOTIN recruits the HCV NS5A protein to autophagosomes for degradation, thereby restricting HCV replication. PMID: 26868272
  2. Scotin plays a role in p53-dependent apoptosis PMID: 12135983
Subcellular Location
Endoplasmic reticulum membrane; Single-pass type I membrane protein. Nucleus membrane.
Protein Families
Shisa family
Database Links

HGNC: 30376

OMIM: 607290

KEGG: hsa:51246

STRING: 9606.ENSP00000296444

UniGene: Hs.414579

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