SHISA5 Antibody

Datasheet

Citations (2)

Code	CSB-PA818677LA01HU
Size	US$166
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Image	Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA818677LA01HU at dilution of 1:100 Immunofluorescence staining of A549 cells with CSB-PA818677LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunoprecipitating SHISA5 in HepG2 whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of CSB-PA818677LA01HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA818677LA01HU (6µg) + HepG2 whole cell lysate (500µg) Lane 3: HepG2 whole cell lysate (10µg)
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Product Details
Citations
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) SHISA5 Polyclonal antibody

Uniprot No.

Q8N114

Target Names

SHISA5

Alternative Names

SHISA5 antibody; SCOTIN antibody; PSEC0133 antibody; Protein shisa-5 antibody; Putative NF-kappa-B-activating protein 120 antibody; Scotin antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Protein shisa-5 protein (56-137AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The SHISA5 Antibody (Product code: CSB-PA818677LA01HU) is Non-conjugated. For SHISA5 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA818677LB01HU	SHISA5 Antibody, HRP conjugated	ELISA
FITC	CSB-PA818677LC01HU	SHISA5 Antibody, FITC conjugated
Biotin	CSB-PA818677LD01HU	SHISA5 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF, IP

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:50-1:500
IP	1:200-1:2000

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Citations

Membrane-tethered SCOTIN condensates elicit an endoplasmic reticulum stress response by sequestering luminal BiP A Jo, M Jung, JY Mun, YJ Kim, JY Yoo,Cell Reports,2025
Intrinsically disordered region-mediated condensation of IFN-inducible SCOTIN/SHISA-5 inhibits ER-to-Golgi vesicle transport N Kim,Developmental cell,2023

Customer Reviews and Q&A

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Target Background

Function

Can induce apoptosis in a caspase-dependent manner and plays a role in p53/TP53-dependent apoptosis.

Gene References into Functions

IFN-beta-induced SCOTIN recruits the HCV NS5A protein to autophagosomes for degradation, thereby restricting HCV replication. PMID: 26868272
Scotin plays a role in p53-dependent apoptosis PMID: 12135983

Subcellular Location

Endoplasmic reticulum membrane; Single-pass type I membrane protein. Nucleus membrane.

Protein Families

Shisa family

Database Links

HGNC: 30376

OMIM: 607290

KEGG: hsa:51246

STRING: 9606.ENSP00000296444

UniGene: Hs.414579

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

p53 signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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