SNX12 Antibody

Datasheet

Code	CSB-PA891787LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: PC-3 whole cell lysate, A549 whole cell lysate All lanes: SNX12 antibody at 2.83µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 20, 19 kDa Observed band size: 20 kDa IHC image of CSB-PA891787LA01HU diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of A549 cells with CSB-PA891787LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunoprecipitating SNX12 in A549 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA891787LA01HU in A549 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA891787LA01HU (6µg) + A549 whole cell lysate (500µg) Lane 3: A549 whole cell lysate (20µg)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) SNX12 Polyclonal antibody

Uniprot No.

Q9UMY4

Target Names

SNX12

Alternative Names

2610001F05Rik antibody; AW045757 antibody; MGC118982 antibody; MGC118983 antibody; RGD1565585 antibody; RP23-356J7.1 antibody; SDP8 antibody; SNX 12 antibody; SNX12 antibody; SNX12_HUMAN antibody; Sorting nexin 12 antibody; Sorting nexin-12 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Sorting nexin-12 protein (2-172AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The SNX12 Antibody (Product code: CSB-PA891787LA01HU) is Non-conjugated. For SNX12 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA891787LB01HU	SNX12 Antibody, HRP conjugated	ELISA
FITC	CSB-PA891787LC01HU	SNX12 Antibody, FITC conjugated
Biotin	CSB-PA891787LD01HU	SNX12 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000
IF	1:200-1:500
IP	1:200-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

May be involved in several stages of intracellular trafficking.

Gene References into Functions

SNX12 plays a key role in intraluminal vesicle formation and in the maturation of a subpopulation of early endosomes into late endosomes, thereby regulating selective endocytic transport of cargo for degradation. PMID: 28705836
SNX12 protein level is dramatically decreased in the brain of Alzheimer's disease patients as compared to that of controls. PMID: 22709416
find that overexpression of SNX12 restores the sorting process in an Hrs knockdown background. Altogether, our data show that despite lower expression level, SNX12 shares redundant functions with SNX3 in the biogenesis of multivesicular endosomes PMID: 22719997
Metallothionein deficient mice have a marked decrease in Snx12 during acute lung injury PMID: 16166738

Subcellular Location

Membrane; Peripheral membrane protein; Cytoplasmic side.

Protein Families

Sorting nexin family

Database Links

HGNC: 14976

OMIM: 300883

KEGG: hsa:29934

STRING: 9606.ENSP00000363392

UniGene: Hs.260750

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Endocytosis

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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