SPDL1 Antibody

Datasheet

Code	CSB-PA842662LA01HU
Size	US$166
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Image	Western blot All lanes: SPDL1 antibody at 4µg/ml Lane 1: Hela whole cell lysate Lane 2: 293T whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: A549 whole cell lysate Lane 5: K562 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 71, 59, 62 kDa Observed band size: 71 kDa Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA842662LA01HU at dilution of 1:100
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) SPDL1 Polyclonal antibody

Uniprot No.

Q96EA4

Target Names

SPDL1

Alternative Names

AA409762 antibody; Arsenite related gene 1 protein antibody; Arsenite-related gene 1 protein antibody; CCDC 99 antibody; Ccdc99 antibody; Coiled coil domain containing 99 antibody; Coiled-coil domain-containing protein 99 antibody; FLJ20364 antibody; FLJ40690 antibody; hSpindly antibody; MGC124977 antibody; Protein Spindly antibody; rhabdomyosarcoma antigen MU RMS 40.4A antibody; Rhabdomyosarcoma antigen MU-RMS-40.4A antibody; Rrhabdomyosarcoma antigen protein MU RMS 40.4A antibody; SPDL1 antibody; SPDLY_HUMAN antibody; Spindle apparatus coiled coil domain containing protein 1 antibody; Spindle apparatus coiled coil protein 1 antibody; Spindly antibody; Spindly homolog antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Protein Spindly protein (1-300AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The SPDL1 Antibody (Product code: CSB-PA842662LA01HU) is Non-conjugated. For SPDL1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA842662LB01HU	SPDL1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA842662LC01HU	SPDL1 Antibody, FITC conjugated
Biotin	CSB-PA842662LD01HU	SPDL1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function

Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment. Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Plays a role in cell migration.

Gene References into Functions

Farnesylation of Spindly is essential for its localization, & thus for proper localization of dynein & dynactin, to prometaphase kinetochores. Both Spindly and CENP-E farnesylation are required for efficient chromosome congression PMID: 25808490
hSpindly is farnesylated in vivo and farnesylation is essential for its interaction with the RZZ complex and hence Kinetochore localization. PMID: 25825516
Spindly is not only required for kinetochore localization of dynein but is a functional component of a mechanism that couples dynein-dependent poleward movement of chromosomes to their efficient attachment to microtubules. PMID: 20427577
dynein-mediated removal of Spindly from microtubule-attached kinetochores, rather than poleward transport per se, is the critical reaction in checkpoint silencing PMID: 20439434
[hSpindly] Localization of human Spindly (hSpindly) to kinetochores is controlled by the Rod/Zw10/Zwilch (RZZ) complex and Aurora B. PMID: 19468067

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Subcellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Nucleus. Cytoplasm, cytoskeleton, spindle pole. Note=Localizes to the nucleus in interphase and to the kinetochore in early prometaphase. Relocalizes to the mitotic spindle pole before metaphase and is subsequently lost from the spindle poles after chromosome congression is completed. Removal of this protein from the kinetochore requires the dynein/dynactin complex.

Protein Families

Spindly family

Database Links

HGNC: 26010

OMIM: 616401

KEGG: hsa:54908

STRING: 9606.ENSP00000265295

UniGene: Hs.368710

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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