SPPL2A Antibody

Datasheet

Code	CSB-PA855050LA01HU
Size	US$166
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Image	Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA855050LA01HU at dilution of 1:100 Immunofluorescent analysis of Hela cells using CSB-PA855050LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) Western Blot Positive WB detected in: Jurkat whole cell lysate All lanes: SPPL2A antibody at 2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 59 kDa Observed band size: 59 kDa
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) SPPL2A Polyclonal antibody

Uniprot No.

Q8TCT8

Target Names

SPPL2A

Alternative Names

SPPL2A antibody; IMP3 antibody; PSL2 antibody; PSEC0147 antibody; Signal peptide peptidase-like 2A antibody; SPP-like 2A antibody; SPPL2a antibody; EC 3.4.23.- antibody; Intramembrane protease 3 antibody; IMP-3 antibody; Presenilin-like protein 2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Signal peptide peptidase-like 2A protein (26-172AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The SPPL2A Antibody (Product code: CSB-PA855050LA01HU) is Non-conjugated. For SPPL2A Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA855050LB01HU	SPPL2A Antibody, HRP conjugated	ELISA
FITC	CSB-PA855050LC01HU	SPPL2A Antibody, FITC conjugated
Biotin	CSB-PA855050LD01HU	SPPL2A Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:2000-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. Functions in FASLG, ITM2B and TNF processing. Catalyzes the intramembrane cleavage of the anchored fragment of shed TNF-alpha (TNF), which promotes the release of the intracellular domain (ICD) for signaling to the nucleus. Also responsible for the intramembrane cleavage of Fas antigen ligand FASLG, which promotes the release of the intracellular FasL domain (FasL ICD). May play a role in the regulation of innate and adaptive immunity. Catalyzes the intramembrane cleavage of the simian foamy virus envelope glycoprotein gp130 independently of prior ectodomain shedding by furin or furin-like proprotein convertase (PC)-mediated cleavage proteolysis.

Gene References into Functions

Study propose that elements within the transmembrane segment and the luminal juxtamembrane domain facilitate intramembrane proteolysis of CD74 by SPPL2a. PMID: 26987812
In human B cells, SPPL2a is indispensable for turnover of CD74 N-terminal fragment. A human 15q21.2 microdeletion leads to loss of SPPL2a transcript and protein. PMID: 25035924
Data show that endogenous SPPL2a - in agreement with overexpression studies - is localised in membranes of lysosomes/late endosomes. PMID: 21896273
ADAM10 and SPPL2a were identified as two proteases implicated in FasL processing and release of the FasL intracellular domain, which has been shown to be important for retrograde FasL signaling PMID: 17557115

Subcellular Location

Late endosome membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Membrane; Multi-pass membrane protein; Lumenal side. Note=Colocalizes with palmitoylated and myristoylated proteins at the plasma membrane.

Protein Families

Peptidase A22B family

Tissue Specificity

Ubiquitous.

Database Links

HGNC: 30227

OMIM: 608238

KEGG: hsa:84888

STRING: 9606.ENSP00000261854

UniGene: Hs.401537

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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