SPRED1 Antibody

Datasheet
Code CSB-PA773600LA01HU
See More Details Free Antibody trial simple
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Uniprot No. Q7Z699
Image
  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate
    All lanes: SPRED1 antibody at 3µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 51 kDa
    Observed band size: 51 kDa

  • Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA773600LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA773600LA01HU at dilution of 1:100

  • Immunofluorescent analysis of A549 cells using CSB-PA773600LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunogen Recombinant Human Sprouty-related, EVH1 domain-containing protein 1 protein (123-311AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Relevance Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase. Negatively regulates hematopoiesis of bone marrow (By similarity).
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Sprouty-related, EVH1 domain-containing protein 1 (Spred-1) (hSpred1), SPRED1
Immunogen Species Human
Research Area Signal Transduction
Target Names SPRED1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase. Negatively regulates hematopoiesis of bone marrow (By similarity).
Involvement in disease Neurofibromatosis 1-like syndrome (NFLS)
Subcellular Location Cell membrane, Peripheral membrane protein, Membrane, caveola, Peripheral membrane protein, Nucleus
Tissue Specificity Weakly expressed in embryonic cell line HEK293.
Database Links

HGNC: 20249

OMIM: 609291

KEGG: hsa:161742

STRING: 9606.ENSP00000299084

UniGene: Hs.525781

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