TAF4B Antibody

Datasheet

Code	CSB-PA849782LA01HU
Size	US$166
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Image	IHC image of CSB-PA849782LA01HU diluted at 1:500 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of A549 cells with CSB-PA849782LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TAF4B Polyclonal antibody

Uniprot No.

Q92750

Target Names

TAF4B

Alternative Names

TAF4B antibody; TAF2C2 antibody; TAFII105 antibody; Transcription initiation factor TFIID subunit 4B antibody; Transcription initiation factor TFIID 105 kDa subunit antibody; TAF(II)105 antibody; TAFII-105 antibody; TAFII105 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Transcription initiation factor TFIID subunit 4B protein (132-244AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The TAF4B Antibody (Product code: CSB-PA849782LA01HU) is Non-conjugated. For TAF4B Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA849782LB01HU	TAF4B Antibody, HRP conjugated	ELISA
FITC	CSB-PA849782LC01HU	TAF4B Antibody, FITC conjugated
Biotin	CSB-PA849782LD01HU	TAF4B Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Cell type-specific subunit of the general transcription factor TFIID that may function as a gene-selective coactivator in certain cells. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAF4B is a transcriptional coactivator of the p65/RELA NF-kappa-B subunit. Involved in the activation of a subset of antiapoptotic genes including TNFAIP3. May be involved in regulating folliculogenesis. Through interaction with OCBA/POU2AF1, acts as a coactivator of B-cell-specific transcription. Plays a role in spermiogenesis and oogenesis.

Gene References into Functions

The existence of a highly conserved TAF4b-dependent gene regulatory network. PMID: 27341508
Two candidate loci in each family and homozygous truncating mutations p.R611X in TAF4B in family 1 and p.K507Sfs*3 in ZMYND15 in family 2, were identified. PMID: 24431330
We show evidence for the first time of an interdependence of TAF4b and AP-1 family members in cell type-specific promoter recognition and initiation of transcription in the context of cancer progression and EMT. PMID: 20353996
histone fold domain mediated interaction enhances the DNA binding activity of each of the TAF6-TAF9 and TAF4b-TAF12 pairs and of a histone-like octamer complex composed of the four TAFs PMID: 15601843
work suggests that pre-mRNA processing and post-translational modification represent two important regulatory steps for the gonad-specific functions of human TAF(II)105 PMID: 16088961
Expression of the TAF4b gene is induced by MYC through a non-canonical, but not canonical, E-box which contributes to its specific response to MYC. PMID: 19020761

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Involvement in disease

Spermatogenic failure 13 (SPGF13)

Subcellular Location

Nucleus. Cytoplasm.

Protein Families

TAF4 family

Tissue Specificity

Preferentially expressed in ovarian granulosa cells (at protein level). Highly expressed in B-cells.

Database Links

HGNC: 11538

OMIM: 601689

KEGG: hsa:6875

STRING: 9606.ENSP00000269142

UniGene: Hs.369519

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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