TAF7L Antibody

Datasheet

Code	CSB-PA688972LA01HU
Size	US$166
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Image	IHC image of CSB-PA688972LA01HU diluted at 1:300 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA688972LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TAF7L Polyclonal antibody

Uniprot No.

Q5H9L4

Target Names

TAF7L

Alternative Names

TAF7L antibody; TAF2Q antibody; Transcription initiation factor TFIID subunit 7-like antibody; Cancer/testis antigen 40 antibody; CT40 antibody; RNA polymerase II TBP-associated factor subunit Q antibody; TATA box-binding protein-associated factor 50 kDa antibody; Transcription initiation factor TFIID 50 kDa subunit antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Transcription initiation factor TFIID subunit 7-like protein (1-98AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The TAF7L Antibody (Product code: CSB-PA688972LA01HU) is Non-conjugated. For TAF7L Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA688972LB01HU	TAF7L Antibody, HRP conjugated	ELISA
FITC	CSB-PA688972LC01HU	TAF7L Antibody, FITC conjugated
Biotin	CSB-PA688972LD01HU	TAF7L Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Probably functions as a spermatogenesis-specific component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. May play a role in spermatogenesis.

Gene References into Functions

Over-expression of TAF7L was observed in breast tumor cell lines. PMID: 26107214
Frameshift mutations of TAF7L gene is associated with stomach and colorectal cancers. PMID: 25098277
expression of AURKC, OIP5, PIWIL2 and TAF7L differed between patients with Acute myeloid leukemia, myelodysplastic syndrome and healthy controls in a gender-dependent manner PMID: 23292864
TAF7L gene polymorphisms were found in male infertility patients and normal controls. PMID: 16597641
The sequence variant in exon 13 of the TAF7L gene located on the X chromosome may represent a risk factor for spermatogenic failure. PMID: 17714218

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Subcellular Location

Nucleus. Cytoplasm.

Protein Families

TAF7 family

Tissue Specificity

Testis-specific.

Database Links

HGNC: 11548

OMIM: 300314

KEGG: hsa:54457

STRING: 9606.ENSP00000361998

UniGene: Hs.223806

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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