TBC1D1 Antibody

Datasheet

Code	CSB-PA803114LA01HU
Size	US$166
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Image	IHC image of CSB-PA803114LA01HU diluted at 1:500 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA803114LA01HU diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TBC1D1 Polyclonal antibody

Uniprot No.

Q86TI0

Target Names

TBC1D1

Alternative Names

KIAA1108 antibody; TBC 1 antibody; TBC antibody; TBC1 (tre 2/USP6; BUB2; cdc16) domain family; member 1 antibody; TBC1 antibody; TBC1 domain family member 1 antibody; Tbc1d1 antibody; TBCD1_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human TBC1 domain family member 1 protein (566-718AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The TBC1D1 Antibody (Product code: CSB-PA803114LA01HU) is Non-conjugated. For TBC1D1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA803114LB01HU	TBC1D1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA803114LC01HU	TBC1D1 Antibody, FITC conjugated
Biotin	CSB-PA803114LD01HU	TBC1D1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:500-1:1000

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

May act as a GTPase-activating protein for Rab family protein(s). May play a role in the cell cycle and differentiation of various tissues. Involved in the trafficking and translocation of GLUT4-containing vesicles and insulin-stimulated glucose uptake into cells.

Gene References into Functions

TBC1D1-Ser(231) phosphorylation and/or 14-3-3 binding partially mediates AMPK-governed glucose homeostasis and muscle glucose uptake in a context-dependent manner. PMID: 27826658
data demonstrate heterozygous deactivating TBC1D1 mutations in CAKUT patients with a similar renal and ureteral phenotype, and provide evidence that TBC1D1 mutations may contribute to CAKUT PMID: 26572137
AS160 and TBC1D1 phosphorylations were evident 30 min after exercise. PMID: 24876356
Data show that TBC1D1 is expressed and phosphorylated in response to glucose in these cells. PMID: 24239544
Moderate association of rs9852 indicates an influence of TBC1D1 for antipsychotic-induced weight gain. PMID: 23364847
R125W mutation occurs in a location of the TBC1D1 PTB domain that is predicted to have a function in a putative protein:protein interaction. PMID: 23667688
Data suggest that insulin increases phosphorylation of signaling nexus TBC1D1 independent of prior exercise or lipid/Intralipid administration. PMID: 22851577
TBC1D1 is Ser237 phosphorylated and binding capacity to 14-3-3 protein is increased in skeletal muscle following exercise. PMID: 20837646
[review] The question of a mechanism for increased phosphorylation of Ser237-TBC1D1 after exercise has, so far, not had a straightforward experimental approach for a direct answer in humans undergoing in vivo exercise. PMID: 21078596
A single bout of exercise regulates TBC1D1 and AS160 phosphorylation on multiple sites in human skeletal muscle. PMID: 21505148
Crystal structures of human TBC1D1 and TBC1D4 (AS160) RabGTPase-activating protein (RabGAP) domains reveal critical elements for GLUT4 translocation. PMID: 21454505
coding variant R125W affects obesity susceptibility, delimits the location of an obesity gene at 4q34-35 and identifies a gene/gene interaction that influences the risk for obesity predisposition PMID: 16893906
form of Tbc1d1 also inhibited GLUT4 translocation and that this effect also required a functional GAP domain PMID: 17274760
These results confirm a putative role of TBC1D1 R125W variant in familial obesity predisposition. PMID: 18325908

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Subcellular Location

Nucleus.

Database Links

HGNC: 11578

OMIM: 609850

KEGG: hsa:23216

STRING: 9606.ENSP00000261439

UniGene: Hs.176503

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

AMPK signaling

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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