Recombinant Human TBC1 domain family member 1 (TBC1D1), partial

Code CSB-YP803114HU
MSDS
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Source Yeast
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Code CSB-EP803114HU
MSDS
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Source E.coli
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Code CSB-EP803114HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP803114HU
MSDS
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Source Baculovirus
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Code CSB-MP803114HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TBC1D1
Uniprot No.
Alternative Names
KIAA1108; TBC 1; TBC; TBC1 (tre 2/USP6; BUB2; cdc16) domain family; member 1; TBC1; TBC1 domain family member 1; Tbc1d1; TBCD1_HUMAN
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May act as a GTPase-activating protein for Rab family protein(s). May play a role in the cell cycle and differentiation of various tissues. Involved in the trafficking and translocation of GLUT4-containing vesicles and insulin-stimulated glucose uptake into cells.
Gene References into Functions
  1. TBC1D1-Ser(231) phosphorylation and/or 14-3-3 binding partially mediates AMPK-governed glucose homeostasis and muscle glucose uptake in a context-dependent manner. PMID: 27826658
  2. data demonstrate heterozygous deactivating TBC1D1 mutations in CAKUT patients with a similar renal and ureteral phenotype, and provide evidence that TBC1D1 mutations may contribute to CAKUT PMID: 26572137
  3. AS160 and TBC1D1 phosphorylations were evident 30 min after exercise. PMID: 24876356
  4. Data show that TBC1D1 is expressed and phosphorylated in response to glucose in these cells. PMID: 24239544
  5. Moderate association of rs9852 indicates an influence of TBC1D1 for antipsychotic-induced weight gain. PMID: 23364847
  6. R125W mutation occurs in a location of the TBC1D1 PTB domain that is predicted to have a function in a putative protein:protein interaction. PMID: 23667688
  7. Data suggest that insulin increases phosphorylation of signaling nexus TBC1D1 independent of prior exercise or lipid/Intralipid administration. PMID: 22851577
  8. TBC1D1 is Ser237 phosphorylated and binding capacity to 14-3-3 protein is increased in skeletal muscle following exercise. PMID: 20837646
  9. [review] The question of a mechanism for increased phosphorylation of Ser237-TBC1D1 after exercise has, so far, not had a straightforward experimental approach for a direct answer in humans undergoing in vivo exercise. PMID: 21078596
  10. A single bout of exercise regulates TBC1D1 and AS160 phosphorylation on multiple sites in human skeletal muscle. PMID: 21505148
  11. Crystal structures of human TBC1D1 and TBC1D4 (AS160) RabGTPase-activating protein (RabGAP) domains reveal critical elements for GLUT4 translocation. PMID: 21454505
  12. coding variant R125W affects obesity susceptibility, delimits the location of an obesity gene at 4q34-35 and identifies a gene/gene interaction that influences the risk for obesity predisposition PMID: 16893906
  13. form of Tbc1d1 also inhibited GLUT4 translocation and that this effect also required a functional GAP domain PMID: 17274760
  14. These results confirm a putative role of TBC1D1 R125W variant in familial obesity predisposition. PMID: 18325908

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Subcellular Location
Nucleus.
Database Links

HGNC: 11578

OMIM: 609850

KEGG: hsa:23216

STRING: 9606.ENSP00000261439

UniGene: Hs.176503

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