TBCB Antibody

Datasheet

Citations (1)

Code	CSB-PA03169A0Rb
Size	US$166
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Image	Western blot All lanes: TBCB antibody at 4µg/ml Lane 1: Hela whole cell lysate Lane 2: A549 whole cell lysate Lane 3: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 28, 22 kDa Observed band size: 28 kDa Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA03169A0Rb at dilution of 1:100 Immunoprecipitating TBCB in Hela whole cell lysate Lane 1: Rabbit control IgG instead of (1µg) instead of CSB-PA03169A0Rb in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: CSB-PA03169A0Rb (4µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg) Immunofluorescent analysis of Hela cells using CSB-PA03169A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
Citations
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TBCB Polyclonal antibody

Uniprot No.

Q99426

Target Names

TBCB

Alternative Names

CG22 antibody; CKAPI antibody; Cytoskeleton associated protein 1 antibody; Cytoskeleton associated protein CKAPI antibody; Cytoskeleton-associated protein 1 antibody; Cytoskeleton-associated protein CKAPI antibody; Tbcb antibody; TBCB_HUMAN antibody; Tubulin folding cofactor B antibody; Tubulin specific chaperone B antibody; Tubulin-folding cofactor B antibody; Tubulin-specific chaperone B antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Tubulin-folding cofactor B protein (1-244AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The TBCB Antibody (Product code: CSB-PA03169A0Rb) is Non-conjugated. For TBCB Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA03169B0Rb	TBCB Antibody, HRP conjugated	ELISA
FITC	CSB-PA03169C0Rb	TBCB Antibody, FITC conjugated
Biotin	CSB-PA03169D0Rb	TBCB Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200
IF	1:50-1:200
IP	1:200-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Citations

Dysregulation of a novel miR-1825/TBCB/TUBA4A pathway in sporadic and familial ALS Anika M. Helferich.et al,Cellular and Molecular Life Sciences,2018

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Applications : WB

Sample type: Human HEK293 cells

Review: The downregulation of TBCB and CASP3 as observed by mass spectrometry could be confirmed in mimic-1825 transfected HEK293 cells by western blotting, while levels of RPAB3 did not change.

By Anonymous

Target Background

Function

Binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer. Involved in regulation of tubulin heterodimer dissociation. May function as a negative regulator of axonal growth.

Gene References into Functions

Data indicate a microRNA-1825/TBCB/TUBA4A pathway as a putative pathogenic cascade in both familial ALS (fALS) and both sporadic (sALS). PMID: 30030593
the role of the human TBCE and TBCB chaperones in alpha-tubulin-beta-tubulin dissociation, was investigated. PMID: 25908846
results support a role for MAP1 proteins in promoting efficient retrograde trafficking of HIV-1 by stimulating the formation of stable microtubules and mediating the association of HIV-1 cores with microtubules. PMID: 25505242
Overexpression of TBCB leads to the decreased localization of p150 to the microtubule network that might result in a functional modulation of this protein complex. PMID: 22777741
TBCB localizes at spindle and midzone microtubules during mitosis. PMID: 22940919
These findings identified TCoB as the third cytoskeleton protein to be nitrated and suggest a previously undescribed mechanism, whereby growth factor signaling may coordinately integrate nitric oxide signaling in the regulation of microtubule dynamics. PMID: 18048340
TBCB functions as a microtubule density regulator in microglia during activation, and provide an insight into understanding of mechanisms controlling microtubule reorganization during microglial transition between phenotypes. PMID: 19038251
Study demonstrates that, unlike its counterpart TBCE, TBCB only moderately destabilizes microtubules; neither TBCB abundance nor microtubule organization or densities are altered in giant axonal neuropathy mutant fibroblasts. PMID: 19168853

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Subcellular Location

Cytoplasm. Cytoplasm, cytoskeleton.

Protein Families

TBCB family

Tissue Specificity

Found in most tissues.

Database Links

HGNC: 1989

OMIM: 601303

KEGG: hsa:1155

STRING: 9606.ENSP00000221855

UniGene: Hs.31053

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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