TCEA3 Antibody

Datasheet

Code	CSB-PA023271LA01HU
Size	US$166
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Image	IHC image of CSB-PA023271LA01HU diluted at 1:600 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA023271LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TCEA3 Polyclonal antibody

Uniprot No.

O75764

Target Names

TCEA3

Alternative Names

Rhabdomyosarcoma antigen MU RMS 40.22 antibody; Tcea3 antibody; TCEA3_HUMAN antibody; TFIIS antibody; TFIIS.H antibody; Transcription elongation factor A (SII) 3 antibody; Transcription elongation factor A protein 3 antibody; Transcription elongation factor A3 antibody; Transcription elongation factor S-II protein 3 antibody; Transcription elongation factor TFIIS.h antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Transcription elongation factor A protein 3 protein (79-169AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The TCEA3 Antibody (Product code: CSB-PA023271LA01HU) is Non-conjugated. For TCEA3 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA023271LB01HU	TCEA3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA023271LC01HU	TCEA3 Antibody, FITC conjugated
Biotin	CSB-PA023271LD01HU	TCEA3 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:200-1:500

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.

Gene References into Functions

Findings reveal a novel mechanism by which p53 utilizes TFIIS.h to selectively promote the transcriptional elongation of the bax gene, upsurging cell death in response to severe DNA damage. PMID: 27005522
TCEA3 attenuates the proliferation and induces apoptosis of gastric cancer cells PMID: 26498664
Tcea3 binds to TGF-beta I receptor and activates TGF-beta signaling pathway. PMID: 23357533
Study demonstrates a strong synergy between hPAF1C and elongation factor SII/TFIIS and an underlying mechanism involving direct hPAF1C-SII interactions and cooperative binding to RNA polymerase II. PMID: 20178742

Subcellular Location

Nucleus.

Protein Families

TFS-II family

Database Links

HGNC: 11615

OMIM: 604128

KEGG: hsa:6920

STRING: 9606.ENSP00000406293

UniGene: Hs.446354

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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