TCHH Antibody

Datasheet

Code	CSB-PA023309OA01HU
Size	US$166
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Image	IHC image of CSB-PA023309OA01HU diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of A549 cells with CSB-PA023309OA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TCHH Polyclonal antibody

Uniprot No.

Q07283

Target Names

TCHH

Alternative Names

220K trichohyalin granular protein antibody; Hair follicle protein AHF antibody; MGC157889 antibody; MGC157890 antibody; RP11-107M16.3 antibody; TCHH antibody; THH antibody; THL antibody; TRHY_HUMAN antibody; Trichohyalin antibody; Trichohyalin granular protein antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Peptide sequence from Human Trichohyalin protein (1922-1941AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The TCHH Antibody (Product code: CSB-PA023309OA01HU) is Non-conjugated. For TCHH Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA023309OB01HU	TCHH Antibody, HRP conjugated	ELISA
FITC	CSB-PA023309OC01HU	TCHH Antibody, FITC conjugated
Biotin	CSB-PA023309OD01HU	TCHH Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Intermediate filament-associated protein that associates in regular arrays with keratin intermediate filaments (KIF) of the inner root sheath cells of the hair follicle and the granular layer of the epidermis. It later becomes cross-linked to KIF by isodipeptide bonds. It may serve as scaffold protein, together with involucrin, in the organization of the cell envelope or even anchor the cell envelope to the KIF network. It may be involved in its own calcium-dependent postsynthetic processing during terminal differentiation.

Gene References into Functions

The difference between the twoinduced pluripotent stem cell(IPSC) clones in TCHH methylation did not have an obvious effect on its expression in 3D human epidermal equivalent, suggesting that differentiation and tissue formation may mitigate variations in the iPSC methylome. PMID: 27460132
To evaluate the relative contribution of these variants, we performed a second genome-wide scan in 709 samples from the Uyghur, an admixed population with both eastern and western Eurasian ancestries. In Uyghurs, both EDAR (rs3827760: P = 1.92 x 10(-12)) and TCHH (rs11803731: P = 1.46 x 10(-3)) are associated with hair straightness, but EDAR (OR 0.415) has a greater effect than TCHH (OR 0.575). PMID: 27487801
Data suggest that an immune response to trichohyalin and K16 may have a role in the pathogenesis of the enigmatic disorder. PMID: 20722389
BMP-4 signalling activates AHF transcription in hair follicles. PMID: 18643848
In human keratinocytes, we found BMP-4 facilitates trichohyalin (THH) transcription, and lamin C plays a key role in the posttranslational stabilization of THH. PMID: 18643848
Common variants in the TCHH gene are associated with straight hair in Europeans. PMID: 19896111

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Involvement in disease

Uncombable hair syndrome 3 (UHS3)

Protein Families

S100-fused protein family

Tissue Specificity

Found in the hard keratinizing tissues such as the inner root sheath (IRS) of hair follicles and medulla, and in the filiform papillae of dorsal tongue epithelium.

Database Links

HGNC: 11791

OMIM: 190370

KEGG: hsa:7062

STRING: 9606.ENSP00000357794

UniGene: Hs.432416

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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