TFB1M Antibody

Datasheet

Code	CSB-PA023420LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: A549 whole cell lysate All lanes: TFB1M antibody at 4.6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa IHC image of CSB-PA023420LA01HU diluted at 1:500 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA023420LA01HU diluted at 1:500 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA023420LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TFB1M Polyclonal antibody

Uniprot No.

Q8WVM0

Target Names

TFB1M

Alternative Names

N'-adenosyl(rRNA) dimethyltransferase 1 antibody; CGI75 antibody; Dimethyladenosine transferase 1; mitochondrial antibody; h-mtTFB antibody; h-mtTFB1 antibody; hmtTFB antibody; hmtTFB1 antibody; hTFB1M antibody; Mitochondrial 12S rRNA dimethylase 1 antibody; Mitochondrial dimethyladenosine transferase 1 antibody; Mitochondrial transcription factor B1 antibody; mtTFB1 antibody; S-adenosylmethionine-6-N' antibody; Tfb1m antibody; TFB1M_HUMAN antibody; Transcription factor B1 mitochondrial antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Dimethyladenosine transferase 1, mitochondrial protein (59-194AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The TFB1M Antibody (Product code: CSB-PA023420LA01HU) is Non-conjugated. For TFB1M Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA023420LB01HU	TFB1M Antibody, HRP conjugated	ELISA
FITC	CSB-PA023420LC01HU	TFB1M Antibody, FITC conjugated
Biotin	CSB-PA023420LD01HU	TFB1M Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity.

Gene References into Functions

Loss of TFB1M results in mitochondrial dysfunction that leads to impaired insulin secretion and diabetes. PMID: 24916378
Deficiency in TFB1M and impaired mitochondrial function contribute to the pathogenesis of type 2 diabetes. PMID: 21195351
The mRNA levels of TFB1M and TFB2M are influenced by endurance training PMID: 19681768
This transcription factor activates transcription of human mitochondrial DNA. PMID: 12068295
Human mitochondrial transcription factor B1 methylates ribosomal RNA at a conserved stem-loop PMID: 12496758
TFB1 interacts with the C-terminal activation region of h-mtTFA and stimulates transcription independently of its RNA methyltransferase activity PMID: 12897151
TFB1M is a nuclear-encoded modifier gene for phenotypic expression of the deafness-associated homoplasmic A1555G mutation in the mitochondrial 12S rRNA gene. PMID: 15110318
Distinct, but possibly coordinated functions of mtTFB1 and mtTFB2 in mitochondrial gene expression and biogenesis. PMID: 17557812
This study suggested that DNA variants in TFB1M did not contribute to the risk for parkinson disease. PMID: 18980857
determined the variation in the TFAM, TFB1M, and TFB2M genes in cardiac hypertrophy PMID: 19096125
rRNA methyltransferase activity is necessary for induction of mitochondrial biogenesis by TFB1M, but not TFB2M. PMID: 19417006

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Involvement in disease

Variations in TFB1M may influence the clinical expression of aminoglycoside-induced deafness caused by the A1555G mutation in the mitochondrial 12S rRNA.

Subcellular Location

Mitochondrion.

Protein Families

Class I-like SAM-binding methyltransferase superfamily, rRNA adenine N(6)-methyltransferase family, KsgA subfamily

Tissue Specificity

Ubiquitously expressed.

Database Links

HGNC: 17037

OMIM: 607033

KEGG: hsa:51106

STRING: 9606.ENSP00000356134

UniGene: Hs.279908

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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