THEMIS2 Antibody

Datasheet

Code	CSB-PA735984LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: A549 whole cell lysate All lanes: THEMIS2 antibody at 4.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 73, 30, 57, 14, 58, 51 kDa Observed band size: 57 kDa IHC image of CSB-PA735984LA01HU diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of NIH/3T3 cells with CSB-PA735984LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) THEMIS2 Polyclonal antibody

Uniprot No.

Q5TEJ8

Target Names

THEMIS2

Alternative Names

Basement membrane induced antibody; Chromosome 1 open reading frame 38 antibody; ICB 1 antibody; ICB1 antibody; Induced by contact to basement membrane 1 protein antibody; Protein ICB 1 antibody; Protein ICB-1 antibody; Protein THEMIS2 antibody; THEMIS2 antibody; THMS2_HUMAN antibody; Thymocyte expressed molecule involved in selection protein 2 antibody; Thymocyte-expressed molecule involved in selection protein 2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Protein THEMIS2 protein (62-177AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The THEMIS2 Antibody (Product code: CSB-PA735984LA01HU) is Non-conjugated. For THEMIS2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA735984LB01HU	THEMIS2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA735984LC01HU	THEMIS2 Antibody, FITC conjugated
Biotin	CSB-PA735984LD01HU	THEMIS2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

May constitute a control point in macrophage inflammatory response, promoting LPS-induced TLR4-mediated TNF production. Determines the threshold for activation of B cells by low-affinity and low-avidity ligands via PLCG2 activation and its downstream pathways.

Gene References into Functions

SNP rs1467465 of the human gene icb-1 might affect susceptibility to ovarian cancer. PMID: 24826199
Loss of icb-1 expression accelerated proliferation of SK-OV-3, OVCAR-3 and OAW-42 cells and led to upregulation of ovarian cancer biomarkers like KLK10 and CLDN16. PMID: 23474491
Data of this study suggest that icb-1 might exert a tumor-suppressor function in breast cancer and that its loss might confer relative resistance of breast cancer cells to apoptotic drugs. PMID: 22565810
Themis2 and Themis1 exhibit the same potential to restore T cell development and T cell receptor-mediated signaling in Themis1-deficient mice. PMID: 22732588
These findings together with the observed co-expression of icb-1 with E-cadherin in breast cancer samples support an important role of the icb-1 gene in cancer cell differentiation. PMID: 21455565
Data show that a highly significant positive correlation of icb-1 transcript levels with c-erbB2 (HER2) expression (p <.0001). PMID: 20569072
Identification of ICB-1 as a new player in endocrine-related cancer. PMID: 19942713
Increased icb-1 mRNA levels could be observed both in monocytic and granulocytic differentiation in maturing HL-60 cells. PMID: 12191572
The novel interferon-gamma inducible gene icb-1 is identified and its effects on the response of ovarian cancer cells to interferon-gamma are described PMID: 16219472
Data suggest that SNP rs1467465 of human gene icb-1 might affect breast cancer susceptibility. PMID: 19308812

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Subcellular Location

Nucleus. Cytoplasm.

Protein Families

Themis family

Tissue Specificity

Expressed in different endometrial adenocarcinoma cell lines and various other cell lines apart from the prostate cell line LNCaP and the ovarian cancer cell line BG1.

Database Links

HGNC: 16839

OMIM: 617856

KEGG: hsa:9473

STRING: 9606.ENSP00000363031

UniGene: Hs.10649

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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