TIMM23 Antibody

Datasheet

Code	CSB-PA023553ESR2HU
Size	$600
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Image	Western Blot Positive WB detected in: JK whole cell lysate(20µg), MCF-7 whole cell lysate(20µg), 293 whole cell lysate(20µg), A549 whole cell lysate(20µg), Hela whole cell lysate(20µg), Mouse Heart tissue lysate(20µg) All lanes: TIMM23 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 300s Immunofluorescence staining of A549 cell with CSB-PA023553ESR2HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TIMM23 Polyclonal antibody

Uniprot No.

O14925

Target Names

TIMM23

Alternative Names

TIMM23 antibody; TIM23 antibody; Mitochondrial import inner membrane translocase subunit Tim23 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Mitochondrial import inner membrane translocase subunit Tim23 protein (1-209AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IF	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The rabbit was immunized with recombinant human TIMM23 protein (1-209aa) to produce the TIMM23 polyclonal antibody, which is an IgG isoform and exhibits a liquid form. Antigen affinity purification was used to purify this antibody. This TIMM23 antibody shows reactivity with the TIMM23 protein from human, mouse, and rat species.

This TIMM23 polyclonal antibody has been validated to recognize the TIMM23 protein in ELISA, WB, and IHC assays. TIMM23 is an essential protein found in the mitochondrial inner membrane, which is involved in the translocation of preproteins into mitochondria. Therefore, this TIMM23 polyclonal antibody may be utilized to investigate the function and localization of the TIMM23 protein and its role in mitochondrial protein import.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.

Gene References into Functions

Data indicate the essential role of the GABP transcription factor in activating translocase of inner mitochondrial membrane 23 TIMM23 and TIMM23B expression. PMID: 29413900
The interaction of presequence peptides with Tim23 intermembrane space domain is weakened in presence of Ca2+ ion, which implicates that Ca2+ ion may play an important role in the protein import by TIM23 complex. PMID: 27178215
Modulating the sorting of nuclear-encoded proteins into mitochondria, mediated by the TIM23 complex, proves therapeutic in both yeast and patient-derived cells exhibiting ATP synthase deficiency. PMID: 25519239
Three distinct Tim23 complexes, namely, B1, B2, and A, are involved in the maintenance of normal mitochondrial function. PMID: 24636990
GST pull-down experiments and tryptophan fluorescence indicated that there is no interaction between Tim23IMS and Tim50IMS PMID: 23811401
MCJ functions as J co-chaperone of the human TIM23 pre-protein translocase. PMID: 23263864
Study establishes an unexpected molecular link between the TIM23 transport machinery and assembly of respiratory-chain complexes that regulate mitochondrial protein synthesis in response to their assembly state. PMID: 23260140
The non-bound hydrophilic domain of the human Tim23 is in a molten globule configuration with marginal stability, existing as a dimer. PMID: 22306744

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Subcellular Location

Mitochondrion inner membrane; Multi-pass membrane protein.

Protein Families

Tim17/Tim22/Tim23 family

Database Links

HGNC: 17312

OMIM: 605034

KEGG: hsa:100287932

STRING: 9606.ENSP00000260867

UniGene: Hs.524308

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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