TMEM165 Antibody

Code CSB-PA023739LA01HU
Size US$166
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  • Western blot
    All lanes: TMEM165 antibody at 8µg/ml
    Lane 1: Jurkat whole cell lysate
    Lane 2: Hela whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 35, 29 kDa
    Observed band size: 35 kDa

  • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA023739LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA023739LA01HU at dilution of 1:100

  • Immunofluorescent analysis of HepG2 cells using CSB-PA023739LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) TMEM165 Polyclonal antibody
Uniprot No.
Target Names
TMEM165
Alternative Names
TMEM165; TPARL; Transmembrane protein 165; Transmembrane protein PT27; Transmembrane protein TPARL
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Transmembrane protein 165 protein (173-228AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The TMEM165 Antibody (Product code: CSB-PA023739LA01HU) is Non-conjugated. For TMEM165 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA023739LB01HU TMEM165 Antibody, HRP conjugated ELISA
FITC CSB-PA023739LC01HU TMEM165 Antibody, FITC conjugated
Biotin CSB-PA023739LD01HU TMEM165 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
May function as a calcium/proton transporter involved in calcium and in lysosomal pH homeostasis. Therefore, it may play an indirect role in protein glycosylation.
Gene References into Functions
  1. High TMEM165 expression is associated with hepatocellular carcinoma. PMID: 30015898
  2. The finding of numerous splice variants could lead to a family of TMEM165 isoforms. PMID: 28088503
  3. Data indicate the Golgi protein transmembrane protein 165 (TMEM165) as a manganese-sensitive protein in mammalian cells. PMID: 28270545
  4. This manuscript is a review of the current state of knowledge on TMEM165 deficiencies in Congenital Disorders of Glycosylation as well as new data on function of TMEM165 and some speculative models on TMEM165/Golgi functions are discussed. PMID: 27401145
  5. Study not only provides novel insights into the molecular causes of glycosylation defects observed in TMEM165-deficient cells but also suggests that TMEM165 is a key determinant for the regulation of Golgi Mn(2+) homeostasis. PMID: 27008884
  6. Antisense oligonucleotide-mediated pseudoexon skipping used in a Golgi-resident protein, a promising treatment option for a specific TMEM165-congenital disorders of glycosylation. PMID: 24720419
  7. Congenital Disorders of Glycosylation disease-causing mutations of TMEM165 changes subcellular localization of the protein. PMID: 23575229
  8. Defects in TMEM165 affect both Ca2+ and pH homeostasis. PMID: 23569283
  9. we identified TMEM165 (also named TPARL) as a gene involved in congenital disorders of glycosylation (CDG). PMID: 22683087

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Involvement in disease
Congenital disorder of glycosylation 2K (CDG2K)
Subcellular Location
Golgi apparatus membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane. Lysosome membrane. Early endosome membrane. Late endosome membrane.
Protein Families
GDT1 family
Tissue Specificity
Ubiquitously expressed.
Database Links

HGNC: 30760

OMIM: 614726

KEGG: hsa:55858

STRING: 9606.ENSP00000370736

UniGene: Hs.479766

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