TRDMT1 Antibody

Datasheet

Code	CSB-PA675694LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Rat heart tissue All lanes: TRDMT1 antibody at 4μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45, 42, 40, 7, 8, 13 kDa Observed band size: 45 kDa IHC image of CSB-PA675694LA01HU diluted at 1:200 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TRDMT1 Polyclonal antibody

Uniprot No.

O14717

Target Names

TRDMT1

Alternative Names

dDNMT antibody; DmMT 2 antibody; DmMT2 antibody; DNA (cytosine 5 ) methyltransferase 2 antibody; DNA (cytosine 5) methyltransferase like protein 2 antibody; DNA (cytosine-5)-methyltransferase-like protein 2 antibody; DNA 5 cytosine methyltransferase antibody; DNA methyltransferase 2 antibody; DNA methyltransferase homolog HsaIIP antibody; DNA MTase homolog HsaIIP antibody; Dnmt 2 antibody; Dnmt2 antibody; M.HsaIIP antibody; MHsaIIP antibody; nmt 2 antibody; nmt2 antibody; OTTHUMP00000045198 antibody; PuMet antibody; RNMT 1 antibody; RNMT1 antibody; TRDMT 1 antibody; TRDMT_HUMAN antibody; TRDMT1 antibody; tRNA (cytosine 5 ) methyltransferase antibody; tRNA (cytosine(38)-C(5))-methyltransferase antibody; tRNA aspartic acid methyltransferase 1 antibody; tRNA aspartic acid methyltransferase 1 variant 1 antibody; tRNA aspartic acid methyltransferase 1 variant 2 antibody; tRNA aspartic acid methyltransferase 1 variant 3 antibody; tRNA aspartic acid methyltransferase 1 variant 4 antibody; tRNA aspartic acid methyltransferase 1 variant 5 antibody; tRNA aspartic acid methyltransferase 1 variant 8 antibody

Raised in

Rabbit

Species Reactivity

Human, Rat

Immunogen

Recombinant Human tRNA (cytosine(38)-C(5))-methyltransferase protein (170-269AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The TRDMT1 Antibody (Product code: CSB-PA675694LA01HU) is Non-conjugated. For TRDMT1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA675694LB01HU	TRDMT1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA675694LC01HU	TRDMT1 Antibody, FITC conjugated
Biotin	CSB-PA675694LD01HU	TRDMT1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp).

Gene References into Functions

Data suggest that, upon HIV-1 infection, DNMT2 is re-localized from the nucleus to cytoplasmic stress granules where DNMT2 methylates HIV-1 messenger RNA; this methylation increases the stability of the HIV-1 RNA genome and up-regulates virus replication; thus, DNMT2 appears to facilitate HIV-1 infection. PMID: 28476776
The strong effect of some of the somatic cancer mutations on DNMT2 activity suggests that these mutations have a functional role in tumorigenesis. PMID: 25747896
Mapped is the tRNA binding site of DNMT2 by systematically mutating surface-exposed lysine and arginine residues to alanine and studying the tRNA methylation activity and binding of the corresponding variants. PMID: 22591353
the role of Dnmt2 in stress granules could represent a primitive cellular defense mechanism against viral infection. PMID: 20864816
identification of residual DNA-(cytosine-C5) methyltransferase activity PMID: 12794065
cDNA microarray analysis identified several genes involved in DNA methylation, such as DNMT2 and DNMT3a that were more highly expressed in LNCaP-r (an androgen sensitive prostate cancer cell line). PMID: 16173030
genetic and biochemical approach revealed that DNMT2 did not methylate DNA but instead methylated aspartic acid transfer RNA (tRNA(Asp)) and that DNMT2 specifically methylated cytosine 38 in the anticodon loop PMID: 16424344
An association study of 45 folate-related genes in spina bifida: Involvement of tRNA aspartic acid methyltransferase 1 (TRDMT1) PMID: 19161160

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Subcellular Location

Cytoplasm.

Protein Families

Class I-like SAM-binding methyltransferase superfamily, C5-methyltransferase family

Tissue Specificity

Ubiquitous. Higher expression in testis, ovary and thymus and at much lower levels in spleen, prostate, colon, small intestine, and peripheral blood leukocytes.

Database Links

HGNC: 2977

OMIM: 602478

KEGG: hsa:1787

STRING: 9606.ENSP00000367030

UniGene: Hs.351665

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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