Product Details

Uniprot No.

Target Names

TRIM16

Alternative Names

EBBP antibody; Estrogen responsive B box protein antibody; Estrogen-responsive B box protein antibody; TRI16_HUMAN antibody; TRIM 16 antibody; TRIM16 antibody; Tripartite motif containing 16 antibody; Tripartite motif containing protein 16 antibody; Tripartite motif-containing protein 16 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Synthesized peptide derived from the N-terminal region of Human TRIM16.

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Form

Liquid

Tested Applications

IHC, ELISA

Recommended Dilution

Application	Recommended Dilution
IHC	1:100-1:300
ELISA	1:10000

Protocols

Immunohistochemistry (IHC) Protocol
ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Related Products

Target Background

Function

E3 ubiquitin ligase that plays an essential role in the organization of autophagic response and ubiquitination upon lysosomal and phagosomal damages. Plays a role in the stress-induced biogenesis and degradation of protein aggresomes by regulating the p62-KEAP1-NRF2 signaling and particularly by modulating the ubiquitination levels and thus stability of NRF2. Acts as a scaffold protein and facilitates autophagic degradation of protein aggregates by interacting with p62/SQSTM, ATG16L1 and LC3B/MAP1LC3B. In turn, protects the cell against oxidative stress-induced cell death as a consequence of endomembrane damage.

Gene References into Functions

High TRIM16 expression is associated with drug resistance in Non-Small Cell Lung Cancer. PMID: 29295721
TRIM16 inhibits the migration and invasion via suppressing the Sonic hedgehog signaling pathway in ovarian cancer cells. PMID: 27737724
The cooperation between TRIM16 and Galectin-3 in targeting and activation of selective autophagy protects cells from lysosomal damage and Mycobacterium tuberculosis invasion. PMID: 27693506
TRIM16 expression is decreased in prostate cancer tissues and overexpression of TRIM16 inhibits cell migration, invasion and the EMT process in vitro in prostate cancer through the transcription factor Snail. PMID: 27748839
Results found that the expression of TRIM16 was significantly downregulated in hepatocellular carcinoma cell (HCC) lesions and define TRIM16 as an inhibitor of epithelial-mesenchymal transition and metastasis in HCC. PMID: 26892350
TRIM16 directly regulated the degradation of Gli1 protein via the ubiquitinproteasome pathway. TRIM16 expression was low in breast cancer. It negatively correlated with metastasis and suppressed stem-cell properties in breast cancer cells. PMID: 26718507
Data suggest that TRIM16 and TDP43 are both good prognosis indicators; data shows that TRIM16 inhibits cancer cell viability by a novel mechanism involving interaction and stabilisation of TDP43 with consequent effects on E2F1 and pRb proteins. PMID: 26902425
Expression of SDMGC and TRIM16 was upregulated in the distant metastasis tissues PMID: 25866896
Chromatin immunoprecipitation assays revealed TRIM16 directly bound the IFNbeta1 gene promoter. Low level TRIM16 expression in 91 melanoma patient samples, strongly correlated with lymph node metastasis, and, predicted poor patient prognosis. PMID: 25333256
results suggest that TRIM16 is a potential pharmacologic target for the treatment of NSCLC and promotion TRIM16 expression might represent a novel strategy to NSCLC metastasis PMID: 25843803
TRIM16 can promote apoptosis by directly modulating caspase-2 activity in cancer cells. PMID: 23404198
data suggest that TRIM16 acts as a novel regulator of both neuroblastoma G 1/S progression and cell differentiation PMID: 23422002
via its unique structure, TRIM16 possesses both heterodimerization function with other TRIM proteins and also has E3 ubiquitin ligase activity. PMID: 22629402
TRIM16 acts as a tumour suppressor, affecting neuritic differentiation, cell migration and replication through interactions with cytoplasmic vimentin and nuclear E2F1 in neuroblastoma cells. PMID: 20729920
role in regulating keratinocyte differentiation PMID: 11919186
These results provide evidence for a role of EBBP in innate immunity by enhancing the alternative secretion pathway of IL-1beta. PMID: 16575408
EBBP increased betaRARE-transactivating function through its coiled-coil domain PMID: 16636064
The estrogen-responsive B box protein (EBBP) restores retinoid sensitivity in retinoid-resistant cancer cells via effects on histone acetylation. PMID: 19147277

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Subcellular Location

Cytoplasm.

Protein Families

TRIM/RBCC family

Tissue Specificity

Highest levels found in testis, ovary, small intestine, colon, placenta, heart, skeletal muscle and mammary gland. More highly expressed in the fetus than in the corresponding adult tissues. Expressed in basal keratinocytes.

Database Links

HGNC: 17241

OMIM: 609505

KEGG: hsa:10626

STRING: 9606.ENSP00000338989

UniGene: Hs.123534