TRIP13 Antibody

Datasheet

Code	CSB-PA623008LA01HU
Size	US$166
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Image	Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA623008LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA623008LA01HU at dilution of 1:100 Western blot All lanes: TRIP13 antibody at 1µg/ml + A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 49, 23 kDa Observed band size: 49 kDa
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TRIP13 Polyclonal antibody

Uniprot No.

Q15645

Target Names

TRIP13

Alternative Names

16E1-BP antibody; 16E1BP antibody; Homo sapiens HPV16 E1 protein binding protein mRNA complete cds antibody; HPV16 E1 protein binding protein antibody; HPV16 E1 protein-binding protein antibody; Human papillomavirus type 16 E1 protein binding protein antibody; Human papillomavirus type 16 E1 protein-binding protein antibody; Pachytene checkpoint protein 2 homolog antibody; PCH2 antibody; Thyroid hormone receptor interactor 13 antibody; Thyroid receptor interacting protein 13 antibody; Thyroid receptor-interacting protein 13 antibody; TR-interacting protein 13 antibody; TRIP-13 antibody; Trip13 antibody; TRP13_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Pachytene checkpoint protein 2 homolog protein (181-390AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The TRIP13 Antibody (Product code: CSB-PA623008LA01HU) is Non-conjugated. For TRIP13 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA623008LB01HU	TRIP13 Antibody, HRP conjugated	ELISA
FITC	CSB-PA623008LC01HU	TRIP13 Antibody, FITC conjugated
Biotin	CSB-PA623008LD01HU	TRIP13 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Plays a key role in chromosome recombination and chromosome structure development during meiosis. Required at early steps in meiotic recombination that leads to non-crossovers pathways. Also needed for efficient completion of homologous synapsis by influencing crossover distribution along the chromosomes affecting both crossovers and non-crossovers pathways. Also required for development of higher-order chromosome structures and is needed for synaptonemal-complex formation. In males, required for efficient synapsis of the sex chromosomes and for sex body formation. Promotes early steps of the DNA double-strand breaks (DSBs) repair process upstream of the assembly of RAD51 complexes. Required for depletion of HORMAD1 and HORMAD2 from synapsed chromosomes. Plays a role in mitotic spindle assembly checkpoint (SAC) activation.

Gene References into Functions

heightened TRIP13 expression appears to promote lung adenocarcinoma tumor progression. PMID: 29567476
TRIP13-p31(comet) intercepts and disassembles free mitotic checkpoint complex not bound to anaphase-promoting complex/cyclosome through mediating the local unfolding of the Mad2 C-terminal region. PMID: 29208896
Microarray analyses indicated that the biological function of TRIP13 in CLL is majorly cell apoptosis and cell proliferation associated. TRIP13 siRNA expressing cells exhibited a slower cell proliferation rate and underwent apoptosis compared with control cells. PMID: 28424416
TRIP13 ubiquitinates and degrades the checkpoint surveillance protein MAD2 via activating Akt signaling pathway, leading to weakened checkpoint surveillance and consequent tumorigenic aneuploidy and drug resistance in MM. PMID: 28157697
These results identify an unexpected dependency on TRIP13 in cells overexpressing Mad2. PMID: 28564602
6 individuals with biallelic loss-of-function mutations in TRIP13 developed Wilms tumors. TRIP13-mutant patient cells have no detectable TRIP13 and have substantial impairment of the spindle assembly checkpoint (SAC), leading to a high rate of chromosome missegregation. Individuals with biallelic TRIP13 mutations have a high risk of embryonal tumors. These experiments show that their cells display severe SAC impairment. PMID: 28553959
Thes authors show that p31(comet) binding to the TRIP13 N-terminal domain positions the disordered MAD2 N-terminus for engagement by the TRIP13 "pore loops", which then unfold MAD2 in the presence of ATP. PMID: 28659378
These results establish a paradigm of the roles of p31(comet) and TRIP13 in both checkpoint activation and inactivation. PMID: 26832417
the oligomeric form of TRIP13 binds both p31(comet) and MCC PMID: 26324890
TRIP13 promotes error-prone non-homologous end joining and induces chemoresistance in head and neck cancer. PMID: 25078033
suggest that premature mitotic checkpoint silencing triggered by TRIP13 overexpression may promote cancer development PMID: 25012665
propose that TRIP13 plays centrally important roles in the sequence of events leading to MCC disassembly and checkpoint inactivation PMID: 25092294

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Involvement in disease

Mosaic variegated aneuploidy syndrome 3 (MVA3)

Protein Families

AAA ATPase family, PCH2 subfamily

Database Links

HGNC: 12307

OMIM: 604507

KEGG: hsa:9319

STRING: 9606.ENSP00000166345

UniGene: Hs.436187

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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