TRO Antibody

Datasheet

Code	CSB-PA025069LA01HU
Size	US$166
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Image	Immunofluorescence staining of Hela cells with CSB-PA025069LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TRO Polyclonal antibody

Uniprot No.

Q12816

Target Names

TRO

Alternative Names

TRO antibody; KIAA1114 antibody; MAGED3 antibody; Trophinin antibody; MAGE-D3 antigen antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Trophinin protein (1-164AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The TRO Antibody (Product code: CSB-PA025069LA01HU) is Non-conjugated. For TRO Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA025069LB01HU	TRO Antibody, HRP conjugated	ELISA
FITC	CSB-PA025069LC01HU	TRO Antibody, FITC conjugated
Biotin	CSB-PA025069LD01HU	TRO Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IF

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200

Protocols

ELISA Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Could be involved with bystin and tastin in a cell adhesion molecule complex that mediates an initial attachment of the blastocyst to uterine epithelial cells at the time of the embryo implantation. Directly responsible for homophilic cell adhesion.

Gene References into Functions

Expression of trophinin and dipeptidyl peptidase IV in endometrial co-culture in the presence of an embryo: A comparative immunocytochemical study. PMID: 27035766
report dual roles for trophinin in human cancers in terms of promoting malignancy in some tumor types and suppressing it in others PMID: 22201876
Trophinin induced apoptosis in endometrial epithelial cells through protein kinase C-delta. PMID: 21191175
infertile women with endometriosis or unexplained infertility had significantly weakened trophinin expression in the endometrium in the mid-luteal phase PMID: 12297480
HCG from the human embryo induces trophinin expression by maternal cells. Unique role of hCG and trophinin in human embryo implantation, including the pathogenesis of ectopic pregnancy. PMID: 14633596
Maged3 expression is restricted to the central nervous system where it was mostly detected in postmitotic neurons PMID: 15162511
Fluorescence photobleaching of GFP-trophinin expressed in COS-1 cells showed the stable association of trophinin with the nuclear envelope. PMID: 16288751
trophinin-mediated cell adhesion functions as a molecular switch for trophectoderm activation in human embryo implantation PMID: 17360433
results suggest that trophinin promotes colorectal cancer invasion through a mechanism involving HMGB1/RAGE PMID: 17487845
Trophinin functions as an adhesion molecule on the cell surface and as a molecular switch for trophoblast activation in the cytoplasm. PMID: 17495530
Confocal microscopy analysis of ectopically expressed magphinins revealed that magphinin-alpha and -beta localize in the cytoplasm, whereas magphinin-gamma lacking the peptide encoded by exon-3 is nuclear. PMID: 17559068
When magphinin-gamma was expressed in NIH3T3 cells, cells underwent G1 arrest. These results suggest that human magphinin-gamma inhibits cell cycle progression through nuclear activity. PMID: 17559068
These findings underscore the importance of TRO in tumorigenesis, and suggest that TRO may be a useful biomarker for cisplatin sensitivity and invasive potential. PMID: 17597582
results provide a mechanism for trophinin-mediated adhesion of human blastocyst to endometrium by a spatially and temporally restricted paracrine effect of hCG derived from the blastocyst PMID: 18078818
Overexpression of trophinin leads to a more invasive phenotype and metastatic potential in human gallbladder cancer transfected into nude mice. PMID: 18846386
TROPHONIN (TRO) is among the genes most downregulated by RAS. TRO expression is higher in cisplatin-sensitive cancer cell lines and positively correlates with prognoses in ovarian cancers. PMID: 17597582

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Tissue Specificity

Strong expression at implantation sites. Found in the placenta from the sixth week of pregnancy. Was localized in the cytoplasm of the syncytiotrophoblast in the chorionic villi and in endometrial decidual cells at the uteroplacental interface. After week

Database Links

HGNC: 12326

OMIM: 300132

KEGG: hsa:7216

STRING: 9606.ENSP00000173898

UniGene: Hs.633653

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