Product Details

Uniprot No.

Target Names

UBE2D1

Alternative Names

E2(17)KB1 antibody; SFT antibody; Stimulator of Fe transport antibody; UB2D1_HUMAN antibody; UBC 4/5 antibody; UBC4/5 antibody; UBC4/5 homolog antibody; UBC4/5, S. cerevisiae, homolog of antibody; UBCH 5 antibody; UBCH 5A antibody; UbcH5 antibody; UBCH5A antibody; Ube2d1 antibody; Ubiquitin carrier protein antibody; Ubiquitin carrier protein D1 antibody; Ubiquitin conjugating enzyme E2 17 kDa 1 antibody; Ubiquitin conjugating enzyme E2 D1 antibody; Ubiquitin conjugating enzyme E2D 1 (UBC4/5 homolog yeast) antibody; Ubiquitin conjugating enzyme E2D 1 antibody; Ubiquitin conjugating enzyme UBCH5A antibody; Ubiquitin protein ligase D1 antibody; Ubiquitin-conjugating enzyme E2 D1 antibody; Ubiquitin-conjugating enzyme E2(17)KB 1 antibody; Ubiquitin-conjugating enzyme E2-17 kDa 1 antibody; Ubiquitin-protein ligase D1 antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Human UBE2D1

Immunogen Species

Homo sapiens (Human)

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.

Tested Applications

ELISA,WB,IHC

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Background

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1. Ubiquitinates STUB1-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4.

Gene References into Functions

The anaphase-promoting complex/cyclosome C activity in human cells is tuned by the combinatorial use of three E2 ubiquitin-conjugating enzymes, namely UBE2C, UBE2S, and UBE2D. PMID: 26904940
Data show that ubiquitin E2 enzymes UBE2D1/2/3 and E3 ligase RNF138 accumulate at DNA-damage sites and act at early resection stages by promoting CtIP protein ubiquitylation and accrual. PMID: 26502057
The Asp17 mutation in MDM2 stimulated its discharge of the UBCH5a-ubiquitin thioester adduct. PMID: 25543083
The SMURF2:UBCH5 complex is critical in maintaining KRAS protein stability. PMID: 24709419
Biochemical characterization of recombinant UBTD1 and UBE2D demonstrated that the two proteins form a stable, stoichiometric complex that can be purified to near homogeneity. PMID: 24211586
crystal structure of the dimeric RING domain of rat RNF4 in complex with E2 (UbcH5A) linked by an isopeptide bond to ubiquitin PMID: 22842904
These data demonstrate that the ability of ICP0 to interact with cellular E2 ubiquitin-conjugating enzyme UBE2D1 is fundamentally important for its biological functions during HSV-1 infection. PMID: 22438555
identify the IDOL-UBE2D complex as an important determinant of LDLR activity, and provide insight into molecular mechanisms underlying the regulation of cholesterol uptake PMID: 21685362
This structure reveals an interaction between the ubiquitin surface flanking K11 and residues adjacent to the E2 catalytic cysteine and suggests a possible role for this surface in formation of K11 linkages. PMID: 21396940
The authors demonstrate that c-IAP1 and UbcH5 family promote K11-linked polyubiquitination of receptor-interacting protein 1 (RIP1) in vitro and in vivo. PMID: 21113135
Whereas UbcH5(A, B and C) is highly efficient in converting IkBa into monoubiquitinated forms, Cdc34 drives ubiquitin (Ub)-Ub conjugation PMID: 20347421
conformational changes in CHIP upon binding of Hsp70, Hsp90, and their respective C-terminal EEVD peptides, and in complex with the different E2 ubiquitin-conjugating enzymes UbcH5a and Ubc13 PMID: 20146531
UbcH5A is up-regulated in the liver of iron-overloaded patients with hereditary hemochromatosis, but in vitro studies failed to show regulation in response to iron loading or chelation. Earlier in vivo mRNA expression data for SFT might be UbcH5A. PMID: 12480712
comparison of the regional distribution of SFT/UbcH5A and DMT1 mRNA in the adult brain PMID: 15139022
Ro52 has both cytoplasmic and nuclear substrates, and mediates ubiquitination through UBE2D1 in the cytoplasm and through UBE2E1 in the nucleus. PMID: 18845142
Catalytically active Ubc5 is required for IRF3 activation by viral infection. PMID: 19854139