Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) UHRF2 Polyclonal antibody

Uniprot No.

Q96PU4

Target Names

UHRF2

Alternative Names

DKFZp434B0920 antibody; DKFZp686G0837 antibody; E3 ubiquitin-protein ligase UHRF2 antibody; MGC33463 antibody; Np95 like RING finger protein antibody; Np95-like ring finger protein antibody; Np95/ICBP90 like RING finger protein antibody; Np95/ICBP90-like RING finger protein antibody; Nuclear protein 97 antibody; Nuclear zinc finger protein Np97 antibody; RING finger protein 107 antibody; RNF 107 antibody; RP11-472F14.2 antibody; Ubiquitin like containing PHD and RING finger domains protein 2 antibody; Ubiquitin-like PHD and RING finger domain-containing protein 2 antibody; Ubiquitin-like-containing PHD and RING finger domains protein 2 antibody; Uhrf2 antibody; UHRF2_HUMAN antibody; URF 2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human E3 ubiquitin-protein ligase UHRF2 protein (444-585AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Biotin

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function

E3 ubiquitin ligase that plays important roles in DNA methylation, histone modifications, cell cycle and DNA repair. Acts as a specific reader for 5-hydroxymethylcytosine (5hmC) and thereby recruits various substrates to these sites to ubiquitinate them. This activity also allows the maintenance of 5mC levels at specific genomic loci and regulates neuron-related gene expression. Participates in cell cycle regulation by ubiquitinating cyclins CCND1 and CCNE1 and thereby inducing G1 arrest. Ubiquitinates also PCNP leading to its degradation by the proteasome. Plays an active role in DNA damage repair by ubiquitinating p21/CDKN1A leading to its proteosomal degradation. Promotes also DNA repair by acting as an interstrand cross-links (ICLs) sensor. Mechanistically, cooperates with UHRF1 to ensure recruitment of FANCD2 to ICLs, leading to FANCD2 monoubiquitination and subsequent activation. Contributes to UV-induced DNA damage response by physically interacting with ATR in response to irradiation, thereby promoting ATR activation.

Gene References into Functions

UHRF2 may be a negative regulator of EMT and a novel prognostic biomarker for ESCC. PMID: 29909415
UHRF2 expression is reduced in some leukemia cell lines, this correlates with promoter hypermethylation, and similar UHRF2 methylation profiles are seen in primary human leukemia samples. Thus, UHRF2 and 5hmC are widely present in differentiated human tissues, and UHRF2 protein is poorly expressed or mislocalized in diverse human cancers. PMID: 27738314
Here, we report the complex structure of PCNA and the peptide ((784)NEILQTLLDLFFPGYSK(800)) derived from UHRF2 that contains a PIP box. Structural analysis combined with mutagenesis experiments provide the molecular basis for the recognition of UHRF2 by PCNA via PIP-box. PMID: 28951215
Results show that UHRF2 down-regulates a number of epithelial-mesenchymal transition markers and up-regulates the expression of transcription factors. The study also suggests that UHRF2 play a role in tumor metastasis. PMID: 27114453
TIP60 acted downstream of UHRF2 to regulate H3K9ac and H3K14ac expression. PMID: 27743347
The overexpression of UHRF2 increased the expression of H3K9ac in L02 normal cells (P<0.01), but decreased the expression of histone H3 lysine 9 acetylation in HepG2 cancer cells. PMID: 28004105
UHRF2 may contribute to the progression of colon carcinogenesis and function as a novel prognostic indicator after curative operation PMID: 24573556
A hydrogen bond between the hydroxyl group of 5hmC and UHRF2-SRA is critical for their preferential binding. PMID: 24813944
UHRF2 is a transcriptional target of E2F, that it directly interacts with E2F1. PMID: 23833190
UHRF2, the homolog of UHRF1, interacts with N-methylpurine DNA glycosylase (MPG) in cancer cells. PMID: 23537643
Low UHRF2 mRNA expression is associated with malignant glioma. PMID: 23244124
UHRF2, a ubiquitin E3 ligase, acts as a small ubiquitin-like modifier E3 ligase for zinc finger protein 131 PMID: 23404503
NIRF may contribute to the coupling between the cell cycle network and the epigenetic landscape PMID: 22673569
In the present study, ubiquitin ligase, NIRF, binds to hepatitis B virus core protein and leads to the proteasome-mediated degradation of hepatitis B virus core protein in vivo. PMID: 22072112
NIRF is immediately adjacent to the single nucleotide polymorphism rs719725, which is reportedly associated with the risk of colorectal cancer. PMID: 21952639
NIRF is frequently upregulated in colorectal cancer and its oncogenicity can be suppressed by let-7a microRNA. PMID: 22018986
the cooperative interplay of Uhrf2 domains may contribute to a tighter epigenetic control of gene expression in differentiated cells PMID: 21598301
NIRF is involved in cell-cycle regulation PMID: 12176013
may participate in the G1/S transition regulation PMID: 15178429
UHRF-2 is an essential molecule for nuclear pQ degradation as a component of nuclear PQC machinery in mammalian cells PMID: 19218238
Growth-inhibitory effect of let-7a on the A549 cells in vitro and in vivo may be explained in part by le-7a-induced suppression of NIRF and elevation of p21(WAF1). PMID: 19818775
Overexpression of NIRF induces cell cycle arrest at G1 phase. NIRF binds to the cyclin E-Cdk2 complex in vivo, and is phosphorylated by Cdk2 in vitro. PMID: 15178429
NIRF is a ubiquitin ligase that is capable of ubiquitinating PCNP (PEST-containing nuclear protein). PMID: 14741369

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Involvement in disease

Associated with various cancers. DNA copy number loss is found in multiple kinds of malignancies originating from the brain, breast, stomach, kidney, hematopoietic tissue and lung.

Subcellular Location

Nucleus. Chromosome.

Database Links

HGNC: 12557

OMIM: 615211

KEGG: hsa:115426

STRING: 9606.ENSP00000276893

UniGene: Hs.493401