Name: USP18 Antibody
Brand: CUSABIO
SKU: CSB-PA891559LA01HU

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) USP18 Polyclonal antibody

Uniprot No.

Q9UMW8

Target Names

USP18

Alternative Names

43 kDa ISG15 specific protease antibody; 43 kDa ISG15-specific protease antibody; EC 3.1.2. antibody; hUBP43 antibody; Interferon Stimulated Gene 43 kD antibody; ISG15 Specific Processing Protease antibody; ISG15-specific-processing protease antibody; ISG43 antibody; Ubiquitin Specific Peptidase 18 antibody; Ubiquitin Specific Protease 18 43 kD antibody; Ubiquitin Specific Protease 18 antibody; Ubl carboxyl terminal hydrolase 18 antibody; Ubl carboxyl-terminal hydrolase 18 antibody; Ubl thioesterase 18 antibody; Ubl thiolesterase 18 antibody; Ubp15 antibody; UBP18_HUMAN antibody; USP18 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Ubl carboxyl-terminal hydrolase 18 protein (18-118AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The USP18 Antibody (Product code: CSB-PA891559LA01HU) is Non-conjugated. For USP18 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA891559LB01HU	USP18 Antibody, HRP conjugated	ELISA
FITC	CSB-PA891559LC01HU	USP18 Antibody, FITC conjugated
Biotin	CSB-PA891559LD01HU	USP18 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The USP18 antibody was derived from the rabbits immunized with the recombinant human USP18 protein (18-118aa). This rabbit anti-human USP18 polyclonal antibody was demonstrated to recognize human, and mouse USP18 proteins in ELISA, WB, IHC, and IF applications. The non-conjugated IgG got purified by protein G and reached up to 95% in purity.

The target protein USP18 mainly regulates the immune response to viral infections and other inflammatory stimuli by deISGylating target proteins and inhibiting the interferon signaling pathway. Mutations in the USP18 gene can lead to autoimmune disorders such as systemic lupus erythematosus (SLE) and type 1 diabetes, as well as susceptibility to viral infections.

Target Background

Function

Involved in the negative regulation of the inflammatory response triggered by type I interferon. Upon recruitment by STAT2 to the type I IFN receptor subunit IFNAR2 interferes with the assembly of the ternary interferon-IFNAR1-IFNAR2 complex and acts as a negative regulator of the type I IFN signaling pathway. Also regulates protein ISGylation. Can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms.; Has enzymatic activity similar to isoform 1 and interferes with type I interferon signaling. Major deISGylation enzyme for nuclear proteins.

Gene References into Functions

These results demonstrate that virus-induced IFN-lambda4 potently blocks IFN-alpha signalling by inducing high protein levels of ISG15 and USP18. Moreover, the data clearly demonstrate that DAA therapy restores IFN-alpha responsiveness in HCV-infected cells. PMID: 28630501
The role of USP18 in breast cancer provides a novel insight into the clinical application of the USP18/AKT/Skp2 pathway. PMID: 29749454
High expression levels of USP18 in the muscles of dermatomyositis patients. PMID: 27605457
study preliminary elucidated the role of USP18 in hepatitis B virus replication and explored the potential key genes in USP18-mediated signaling pathway PMID: 28369997
this study identified PTEN as a previously unrecognized substrate of the ISGylation post-translational modification pathway. The deconjugase USP18 serves as a novel regulator of PTEN stability. This indicates inhibition of ISGylation is therapeutically relevant in cancers. PMID: 27980214
associates with and deubiquitinates TAK1 to protect against hepatic steatosis, insulin resistance, and the inflammatory response PMID: 28718215
that Ubiquitin-specific peptidase 18 directly bind to BCL2L1 and positively regulated its expression in hepatocellular carcinoma cells PMID: 28709980
These findings add USP18 deficiency to the list of genetic disorders collectively termed type I interferonopathies PMID: 27325888
We show that IRF-7 siRNA knockdown enhanced LPS-induced IL-10 production in human monocyte-derived macrophages, and USP-18 overexpression attenuated LPS-induced production of IL-10 in RAW264.7 cells. Quantitative PCR confirmed upregulation of USP18, USP41, IL10, and IRF7. An independent cohort confirmed LPS induction of USP41 and IL10 genes PMID: 27434537
results indicated that USP18 modulates the anti-HBV activity of IFN-F via activation of the JAK/STAT signaling pathway in Hepg2.2.15 cells. PMID: 27227879
USP18's ISG15 specificity is mediated by a small interaction interface. PMID: 28165509
STAT2 recruits USP18 to the type I IFN receptor subunit IFNAR2 via its constitutive membrane-distal STAT2-binding site. PMID: 28165510
findings show that multiple inflammatory stimuli can modulate interferon stimulated gene expression and thus inhibit hepatocyte interferon signaling via USP18 induction PMID: 27009955
USP18 negatively regulates NF-kappaB signaling by targeting TAK1 and NEMO for deubiquitination through distinct mechanisms. PMID: 26240016
Data suggest that USP18 (Ubiquitin-like specific protease 18) sensitive cellular functions include activity of the peptide transporters PEPT1 and PEPT2. PMID: 26046984
Dimerization of IFNAR1 and IFNAR2 and the limiting role of IFNAR1 binding affinity in complex assembly is modulated by USP18. PMID: 26008745
increased expression in the HIV+/HCV+ female patients group compared with HIV-/HCV+ and HIV+/HCV- groups PMID: 24955730
USP18 expression levels induced by IFNbeta did not differ amongst multiple sclerosis patients carrying different rs2542109 genotypes PMID: 23700969
USP18 inhibition induces inflammation by increasing the STAT signaling and exacerbates IFN-induced beta cell apoptosis. PMID: 23152055
Usp18 upregulation was associated with Wilms Tumor. PMID: 23291318
A direct relationship was found between UBP43 and cyclin D1 (but not cyclin E) expression. PMID: 22752428
USP18 establishes the transcriptional and anti-proliferative interferon alpha/beta differential PMID: 22731491
USP18 is up-regulated in liver samples of patients with chronic hepatitis C that did not respond to therapy, but not in patients with acute hepatitis C. PMID: 22677194
although UBP43 depletion can cause hypersensitivity to interferon-alpha/beta-mediated apoptosis in a broad range of cell types, the downstream pathway may vary depending on the cell type. PMID: 22683641
the existence of an N-terminal truncated isoform of USP18, whose expression is controlled on translational level by two independent mechanisms providing translational flexibility PMID: 22170061
Inhibition of R. conorii-induced ISG15 by RNA interference results in significant increase in the extent of rickettsial replication, whereas UBP43 knockdown yields a reciprocal inhibitory effect. PMID: 22100648
a model in which Usp18 inhibition promotes up-regulation of miR-7, which in turn inhibits EGFR expression and the tumorigenic activity of cancer cells. PMID: 21592959
Findings define the ubiquitin protease UBP43 as a novel candidate drug target for APL treatment. PMID: 20935222
Findings suggest that USP18 may significantly limit operation of the extrinsic apoptotic pathway triggered by type I IFN and drugs. PMID: 20068173
UBP43 protein levels are regulated by proteolysis via the SCFSkp2 ubiquitin ligase PMID: 15342634
Ubp43 deficiency increases the resistance to oncogenic transformation by BCR-ABL PMID: 17374743
Epstein-Barr virus independent dysregulation of UBP43 expression alters interferon-stimulated gene expression in Burkitt lymphoma PMID: 19551150

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Involvement in disease

Pseudo-TORCH syndrome 2 (PTORCH2)

Subcellular Location

[Isoform 1]: Cytoplasm.; [Isoform 2]: Nucleus. Cytoplasm.

Protein Families

Peptidase C19 family

Database Links

HGNC: 12616

OMIM: 607057

KEGG: hsa:11274

STRING: 9606.ENSP00000215794

UniGene: Hs.38260

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Code	CSB-PA891559LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: MCF-7 whole cell lysate, Mouse skeletal muscle tissue All lanes: USP18 antibody at 2μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44, 42 kDa Observed band size: 44 kDa Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA891559LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA891559LA01HU at dilution of 1:100 Immunofluorescent analysis of MCF-7 cells using CSB-PA891559LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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USP18 Antibody

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Target Background