VPS25 Antibody

Datasheet

Code	CSB-PA887108LA01HU
Size	US$166
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Image	Western blot All lanes: VPS25 antibody at 3µg/ml Lane 1: Mouse kidney tissue Lane 2: HL60 whole cell lysate Lane 3: THP-1 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 21 kDa Observed band size: 21 kDa Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA887108LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA887108LA01HU at dilution of 1:100 Immunofluorescence staining of Hela cells with CSB-PA887108LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) VPS25 Polyclonal antibody

Uniprot No.

Q9BRG1

Target Names

VPS25

Alternative Names

Dermal papilla derived protein 9 antibody; Dermal papilla-derived protein 9 antibody; DERP9 antibody; EAP20 antibody; ELL associated protein of 20 kDa antibody; ELL-associated protein of 20 kDa antibody; ESCRT-II complex subunit VPS25 antibody; FAP20 antibody; HGNC:28122 antibody; hVps25 antibody; MGC10540 antibody; Vacuolar protein sorting 25 homolog (S. cerevisiae) antibody; Vacuolar protein sorting associated protein 25 antibody; Vacuolar protein-sorting-associated protein 25 antibody; VPS25 antibody; VPS25_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Vacuolar protein-sorting-associated protein 25 protein (1-176AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The VPS25 Antibody (Product code: CSB-PA887108LA01HU) is Non-conjugated. For VPS25 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA887108LB01HU	VPS25 Antibody, HRP conjugated	ELISA
FITC	CSB-PA887108LC01HU	VPS25 Antibody, FITC conjugated
Biotin	CSB-PA887108LD01HU	VPS25 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses.

Gene References into Functions

mammalian ESCRTII is made up of hVps25p, hVps22p, and hVps36p and may be redundant, cargo-specific, or not required for protein sorting at the multivesicular body PMID: 16371348

Subcellular Location

Cytoplasm. Endosome membrane. Nucleus, nucleoplasm. Note=Distributes diffusely throughout the cytoplasm and nucleoplasm, but exhibits a punctate distribution on coexpression with CHMP6.

Protein Families

VPS25 family

Tissue Specificity

Expressed at the mRNA level in kidney, liver, pancreas, and placenta. Lower levels of expression are found in heart, skeletal muscle, brain and lung.

Database Links

HGNC: 28122

OMIM: 610907

KEGG: hsa:84313

STRING: 9606.ENSP00000253794

UniGene: Hs.500165

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Endocytosis

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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